玉米阳离子/质子逆向转运蛋白ZmNHX7的功能鉴定
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张凌霄,焦珍珍,卜华虎,王逸茹,李健,郑军
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Functional identification of maize cation/proton antiporter ZmNHX7
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Ling-Xiao ZHANG,Zhen-Zhen JIAO,Hua-Hu BU,Yi-Ru WANG,Jian LI,Jun ZHENG
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表1 引物序列
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Table 1 Primer sequences
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引物用途Purpose of primer | 引物名称Primer name | 引物序列Primer sequence (5'-3') | 扩增基因引物 | ZmNHX7-G-F1 | ATGGGCGGCGAGGCTGAGCC | Amplified gene primer sequences | ZmNHX7-G-R1 | CTACTGCTCCTGGGGCGGAG | | ZmNHX7-G-F2 | GCTGTGGTTGCACTGCTAAA | | ZmNHX7-G-R2 | TGCAATAACAACCCCACTCA | | AtNHX8-G-F | TTCCGTACACCGTCGTTCT | | AtNHX8-G-R | CCCCATCAATTAACGTGGTC | | At-actin-F | GCCAATCCGGTGCTGGTAACA | | At-actin-R | CATACCAGATCCAGTTCCTCCTCCC | 荧光定量PCR引物序列 | ZmNHX7-Q-F | TGGGTTGGACTTGAAAGAGG | RT-PCR primer sequences | ZmNHX7-Q-R | AACACAATGCCACCAGTGAA | | GAPDH-F | AGGATATCAAGAAAGCTATTAAGGC | | GAPDH-R | GTAGCCCCACTCGTTGTCG | 基因亚细胞定位 | ZmNHX7-L-F | AGCAGGCTTTGACTTTATGGGCGGCGAGGCTGAGCCTGACA | Subcellular localization of gene | ZmNHX7-L-R | TGGGTCTAGAGACTTTCTACTGCTCCTGGGGCGGAGGCACG |
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