紫苏二酰基甘油酰基转移酶2基因克隆与功能研究

鲁庚,唐鑫,陆俊杏,李丹,胡秋芸,胡田,张涛

Cloning and function analysis of a type 2 diacylglycerol acyltransferase (DGAT2) from Perilla frutescens

Geng LU,Xin TANG,Jun-Xing LU,Dan LI,Qiu-Yun HU,Tian HU,Tao ZHANG

表1 PCR引物

Table 1 Primers used for PCR

引物名称 Primer name 引物序列 Sequences (5'-3') PfDGAT2 F CTCGCTTACTGCTACTTCAATG PfDGAT2 R CGACAATTAGAGAATCCTGAGC PfDGAT2-DL F AGTCCGAGCCCAACGGCGATGTCAG PfDGAT2-DL R GGATGCCCAACCCTTGCTTTGTGCC Actin F AGACCTTCAATGTGCCAGCCA Actin R CACGACCAGCAAGATCCAACC 18S RNAF CGGCGACGCATCATTCAAA DGAT2-BglII F ACTCTTGACCATGGTAGATCTGGAGTCCGAGCCCAACGGCG DGAT2-BglII R GGACGTAAACTAGTCAGATCTAGAATCCTGAGCTCTAAGTCG JD-F TTTCATTTGGAGAGAACACGGGGGA JD-R CGCTGATCAATTCCACAGTTTTCGC