大豆生物钟基因GmLNK1/2、GmRVE4/8和GmTOC1 CRISPR/Cas9组织表达分析及敲除靶点的鉴定

甘卓然,石文茜,黎永力,侯智红,李海洋,程群,董利东,刘宝辉,芦思佳

Identification of CRISPR/Cas9 knockout targets and tissue expression analysis of circadian clock genes GmLNK1/2, GmRVE4/8, and GmTOC1 in soybean

Zhuo-Ran GAN,Wen-Qian SHI,Yong-Li LI,Zhi-Hong HOU,Hai-Yang LI,Qun CHENG,Li-Dong DONG,Bao-Hui LIU,Si-Jia LU

表2 萌发培养基与根诱导培养基

Table 2 Germination medium and root medium

培养基类型 Medium type 药品名称 Name of the medicine 药品用量 Medicine dosage 萌发培养基 Germination medium B5盐 B5 salt mixture 1× 蔗糖 Sucrose (g L-1) 20 琼脂 Agar (g L-1) 8 发根培养基 Rooting medium MS合成盐 MS salt mixture 1× 蔗糖 Sucrose (g L-1) 30 2-(4-吗啉)乙磺酸 MES 0.6 琼脂 Agar (g L-1) 8 头孢霉素 Cef (mg L-1) 250 羧苄青霉素 Car (mg L-1) 250