大豆生物钟基因GmLNK1/2、GmRVE4/8和GmTOC1 CRISPR/Cas9组织表达分析及敲除靶点的鉴定

甘卓然,石文茜,黎永力,侯智红,李海洋,程群,董利东,刘宝辉,芦思佳

Identification of CRISPR/Cas9 knockout targets and tissue expression analysis of circadian clock genes GmLNK1/2, GmRVE4/8, and GmTOC1 in soybean

Zhuo-Ran GAN,Wen-Qian SHI,Yong-Li LI,Zhi-Hong HOU,Hai-Yang LI,Qun CHENG,Li-Dong DONG,Bao-Hui LIU,Si-Jia LU

图1 同源性比对 A: AtLNK1与GmLNK1a、GmLNK1b、GmLNK1c、GmLNK1d氨基酸序列对比; B: AtLNK2与GmLNK2a、GmLNK2b、GmLNK2c、GmLNK2d氨基酸序列对比; C: AtTOC1与GmTOC1a、GmTOC1b、GmTOC1c、GmTOC1d氨基酸序列对比; D: AtRVE4、AtRVE8与GmRVE4/8a、GmRVE4/8b、GmRVE4/8c、GmRVE4/8d氨基酸序列对比。

Fig. 1 Homology comparison A: amino acid sequences comparison of AtLNK1, GmLNK1a, GmLNK1b, GmLNK1c, and GmLNK1d; B: amino acid sequences comparison of AtLNK2, GmLNK2a, GmLNK2b, GmLNK2c, and GmLNK2d; C: amino acid sequences comparison of AtTOC1, GmTOC1a, GmTOC1b, GmTOC1c, and GmTOC1d; D: amino acid sequences comparison of AtRVE4, AtRVE8, GmRVE4/8a, GmRVE4/8b, GmRVE4/8c, and GmRVE4/8d.