大豆生物钟基因GmLNK1/2、GmRVE4/8和GmTOC1 CRISPR/Cas9组织表达分析及敲除靶点的鉴定

甘卓然,石文茜,黎永力,侯智红,李海洋,程群,董利东,刘宝辉,芦思佳

Identification of CRISPR/Cas9 knockout targets and tissue expression analysis of circadian clock genes GmLNK1/2, GmRVE4/8, and GmTOC1 in soybean

Zhuo-Ran GAN,Wen-Qian SHI,Yong-Li LI,Zhi-Hong HOU,Hai-Yang LI,Qun CHENG,Li-Dong DONG,Bao-Hui LIU,Si-Jia LU

图2 4个生物钟基因组织特异表达 A: GmLNK1a/b/c/d在W82中的组织特异表达; B: GmLNK2a/b/c/d在W82中的组织特异表达; C: GmTOC1a/b/c/d在W82中的组织特异表达; D: GmRVE4/8a/b/c/d在W82中的组织特异表达。

Fig. 2 Expression of four clock genes in soybean tissues A: the expression of GmLNK1a/b/c/d in soybean W82 tissues; B: the expression of GmLNK2a/b/c/d in soybean W82 tissues; C: the expression of GmTOC1a/b/c/d in soybean W82 tissues; D: the expression of GmRVE4/8a/b/c/d in soybean W82 tissues.