大豆生物钟基因GmLNK1/2、GmRVE4/8和GmTOC1 CRISPR/Cas9组织表达分析及敲除靶点的鉴定

甘卓然,石文茜,黎永力,侯智红,李海洋,程群,董利东,刘宝辉,芦思佳

Identification of CRISPR/Cas9 knockout targets and tissue expression analysis of circadian clock genes GmLNK1/2, GmRVE4/8, and GmTOC1 in soybean

Zhuo-Ran GAN,Wen-Qian SHI,Yong-Li LI,Zhi-Hong HOU,Hai-Yang LI,Qun CHENG,Li-Dong DONG,Bao-Hui LIU,Si-Jia LU

图3 Cas9载体结构图 a: 靶点LNK1T1、LNK2T1、TOC1T1和RVE4/8T1; b: 靶点LNK1T2、LNK2T2、TOC1T2和RVE4/8T2; c: 靶点LNK1T3、LNK2T3、TOC1T3和RVE4/8T3; d: 靶点LNK1T4、LNK2T4、TOC1T4和RVE4/8T4。

Fig. 3 Schematic diagram of the Cas9 vector construction a: target of LNK1T1, LNK2T1, TOC1T1, and RVE4/8T1; b: target of LNK1T2, LNK2T2, TOC1T2, and RVE4/8T2; c: target of LNK1T3, LNK2T3, TOC1T3, and RVE4/8T3; d: target LNK1T4, LNK2T4, TOC1T4, and RVE4/8T4.