大豆生物钟基因GmLNK1/2、GmRVE4/8和GmTOC1 CRISPR/Cas9组织表达分析及敲除靶点的鉴定

甘卓然,石文茜,黎永力,侯智红,李海洋,程群,董利东,刘宝辉,芦思佳

Identification of CRISPR/Cas9 knockout targets and tissue expression analysis of circadian clock genes GmLNK1/2, GmRVE4/8, and GmTOC1 in soybean

Zhuo-Ran GAN,Wen-Qian SHI,Yong-Li LI,Zhi-Hong HOU,Hai-Yang LI,Qun CHENG,Li-Dong DONG,Bao-Hui LIU,Si-Jia LU

图4 发生编辑靶点的测序峰图 A: GmLNK1a的靶点LNK1T1、GmLNK1b的靶点LNK1T2、GmLNK1c的靶点LNK1T4和GmLNK1d的靶点LNK1T4突变情况; B: GmLNK2a的靶点LNK2T1、GmLNK2b的靶点LNK2T1、GmLNK2c的靶点LNK2T4和GmLNK2d的靶点LNK2T4突变情况; C: GmTOC1b的靶点TOC1T2、GmTOC1c的靶点TOC1T4和GmTOC1d的靶点TOC1T4突变情况; D: GmRVE4/8b的靶点RVE4/8T1和GmRVE4/8d的靶点RVE4/8T4突变情况。

Fig. 4 Detailed sequence of the targets site in the transgenic soybean hairy roots A: mutations were found in target LNK1T1 of GmLNK1a, target LNK1T2 of GmLNK1b, target LNK1T4 of GmLNK1c and target LNK1T4 of GmLNK1d; B: mutations were found in target LNK2T1 of GmLNK2a, target LNK2T1 of GmLNK2b, target LNK2T4 of GmLNK2c and target LNK2T4 of GmLNK2d; C: mutations were found in target TOC1T2 of GmTOC1b, target TOC1T4 of GmTOC1c and target TOC1T4 of GmTOC1d; D: mutations were found in target RVE4/8T1 of GmRVE4/8b and target RVE4/8T4 of GmRVE4/8d.