芥菜型油菜BjuB.KAN4基因调控类黄酮的途径

高国应, 伍小方, 黄伟, 周定港, 张大为, 周美亮, 张凯旋, 严明理

Regulation of flavonoid pathway by BjuB.KAN4 gene in Brassica juncea

Guo-Ying GAO, Xiao-Fang WU, Wei HUANG, Ding-Gang ZHOU, Da-Wei ZHANG, Mei-Liang ZHOU, Kai-Xuan ZHANG, Ming-Li YAN

表1 引物序列

Table 1 Primer sequences

引物名称 Primer name 引物序列 Primer sequences (5°-3°) 引物用途 Purpose of primer KAN4-B-F AGTGAGATGATCATGTTCGAGTC 基因克隆Gene cloning KAN4-B-R CAATTAGCACTTGAGAAGGGTTA KB-NcoIF GGGGACTCTTGACCATGGTAATGATCATGTTCGAG KAN4载体构建引物 KAN4 vector primers KB-Eco91IR GAAATTCGAGCTGGTCACCCAATTAGCACTTGAGAAGGG KB-pro-BamHIF GGATCCATTGTCGTTGGTGACAGAAAC KAN4启动子载体构建引物 KB-pro vector primer KB-pro-NcoIR CCATGGTTGGAGTTTTCAGAACTTTGGC Actin7-F GCTGACCGTATGAGCAAAG qRT-PCR检测引物 qRT-PCR detection primers Actin7-R AAGATGGATGGACCCGAC qKAN4-B-F AGGACCCAAAGATCTCCTTGGT qKAN4-B-R TTACACATAGTCAATCCCCCAACT qPAL-F AGAGCTTTTGACCGGAGAGA qPAL-R TTAATCACTCTTAACATATAGGAATGGGAG qCHS-F TCTTCATATTGGACGAGATGAGGA qCHS-R GCGTTTCTGTTCAAACAGGAA qCHI-F CTTTGGAGCGACCATTAGAG qCHI-R AGACAAAGCTTAACAAGAGAGGT qF3’H-F TGATTGGGAATTAGCTGGAGGA qF3’H-R AGTTAAATTTTAACCCGACCCGA qF3H-F ATCTTGGAGGAGCCAATTACGT qF3H-R ACACAAGGAGTCTAAGCGATGA qFLS-F ACTAGGAATGTGATCGCACCA qFLS-R TCAGAGGGATTAGGTTTACGG qDFR-F TCTTTGGAACAGGTTTGAAGGA qDFR-R TAAAGTGACAGGGAGAAAACCCT qANS-F AAGCCGTTGCCTGAGA qANS-R AGAGTTTCAGACTCAGACTTCA qBAN-F GGTTTTTGTTGTTAGGGAAAGA qBAN-R ATATGCTTACTCTGACAAAACAT