芥菜型油菜<i>BjuB.KAN4</i>基因调控类黄酮的途径

芥菜型油菜BjuB.KAN4基因调控类黄酮的途径

高国应¹^,²^,³(

), 伍小方¹^,²^,³, 黄伟¹^,³, 周定港¹^,³, 张大为¹^,³, 周美亮², 张凯旋²^,^*(

), 严明理¹^,³^,^*(

)

Regulation of flavonoid pathway by BjuB.KAN4 gene in Brassica juncea

Guo-Ying GAO¹^,²^,³(

), Xiao-Fang WU¹^,²^,³, Wei HUANG¹^,³, Ding-Gang ZHOU¹^,³, Da-Wei ZHANG¹^,³, Mei-Liang ZHOU², Kai-Xuan ZHANG²^,^*(

), Ming-Li YAN¹^,³^,^*(

)

图3. 芥菜型油菜毛状根的诱导过程和转基因根系鉴定
A: 芥菜型油菜毛状根的诱导过程。B: PM和SY转基因毛状根DNA鉴定; PKB1-PKB3: PM阳性毛状根; SKB1-SKB3: SY阳性毛状根; -: H₂O (阴性对照); +: 质粒(阳性对照)。C: PM毛状根中BjuB.KAN4的相对表达量。D: SY毛状根中BjuB.KAN4的相对表达量。A4: A4侵染的毛状根; 3301: 转pCAMBIA3301空载体毛状根; KAN4-B1~B3: 转基因毛状根。**表示在0.01水平差异显著。

Fig. 3. Induction of hairy roots in Brassica juncea and identification of transgenic hairy roots
A: induction process of hairy roots in Brassica juncea. B: DNA identification of PM and SY transgenic hairy roots; PKB1-PKB3: positive hairy roots of PM; SKB1-SKB3: positive hairy roots of SY; -: H₂O (negative control); +: plasmid (positive control). C: relative expression of BjuB.KAN4 in hairy roots of PM. D: relative expression of BjuB.KAN4 in hairy roots of SY. A4: hairy roots infected by A4; 3301: hairy roots transfected with pCAMBIA3301 empty vector; KAN4-B1-B3: transgenic hairy roots. ** Significant at P < 0.01.