芥菜型油菜<i>BjuB.KAN4</i>基因调控类黄酮的途径

芥菜型油菜BjuB.KAN4基因调控类黄酮的途径

高国应¹^,²^,³(

), 伍小方¹^,²^,³, 黄伟¹^,³, 周定港¹^,³, 张大为¹^,³, 周美亮², 张凯旋²^,^*(

), 严明理¹^,³^,^*(

)

Regulation of flavonoid pathway by BjuB.KAN4 gene in Brassica juncea

Guo-Ying GAO¹^,²^,³(

), Xiao-Fang WU¹^,²^,³, Wei HUANG¹^,³, Ding-Gang ZHOU¹^,³, Da-Wei ZHANG¹^,³, Mei-Liang ZHOU², Kai-Xuan ZHANG²^,^*(

), Ming-Li YAN¹^,³^,^*(

)

图4. 转基因毛状根中类黄酮代谢途径关键酶基因的相对表达量
A: PM转基因毛状根; B: SY转基因毛状根。A4: A4侵染的毛状根; 3301: 转pCAMBIA3301空载体毛状根; KAN4-B04: 转基因毛状根。内参基因: Actin7, 每组数据代表3次生物学重复的平均值±SD。PAL: 苯丙氨酸脱氨酶; CHS: 查尔酮合成酶; CHI: 查尔酮异构酶; F3H: 黄烷酮3-羟化酶; F3’H: 类黄酮3’-羟化酶; FLS: 黄酮醇合酶; DFR: 二氢黄酮醇还原酶; ANS: 花青素合成酶; BAN: 花色素还原酶。 *表示在0.05水平差异显著; **表示在0.01水平差异。

Fig. 4. Relative expression of the key enzyme genes involved in flavonoids biosynthesis pathway in transgenic hairy roots
A: transgenic hairy roots of PM; B: transgenic hairy roots of SY. A4: hairy roots infected by A4; 3301: hairy roots transfected with pCAMBIA3301 empty vector; KAN4-B04: transgenic hairy roots. internal control: Actin7, error bars represent the standard deviation of triplicate runs for qRT-PCR. PAL: phenylalanine deaminase; CHS: chalcone synthase; CHI: chalcone isomerase; F3H: flavone 3-hydroxylase; F3’H: flavonoid 3’-hydroxylase; FLS: flavonol synthase; DFR: dihydroflavonol reductase; ANS: anthocyanin synthase; BAN: anthocyanin reductase. * Significant at P < 0.05; ** Significant at P < 0.01.