芥菜型油菜BjuB.KAN4基因调控类黄酮的途径
高国应1,2,3(
), 伍小方1,2,3, 黄伟1,3, 周定港1,3, 张大为1,3, 周美亮2, 张凯旋2,*(), 严明理1,3,*()
), 伍小方1,2,3, 黄伟1,3, 周定港1,3, 张大为1,3, 周美亮2, 张凯旋2,*(), 严明理1,3,*()
Regulation of flavonoid pathway by BjuB.KAN4 gene in Brassica juncea
Guo-Ying GAO1,2,3(), Xiao-Fang WU1,2,3, Wei HUANG1,3, Ding-Gang ZHOU1,3, Da-Wei ZHANG1,3, Mei-Liang ZHOU2, Kai-Xuan ZHANG2,*(), Ming-Li YAN1,3,*()
), Xiao-Fang WU1,2,3, Wei HUANG1,3, Ding-Gang ZHOU1,3, Da-Wei ZHANG1,3, Mei-Liang ZHOU2, Kai-Xuan ZHANG2,*(), Ming-Li YAN1,3,*()
图6. 过表达BjuB.KAN4基因拟南芥分子鉴定
A: 转基因拟南芥阳性PCR鉴定; B: 转基因拟南芥中BjuB.KAN4的相对表达量检测。WT: 野生型拟南芥; KB-1-6: 转基因阳性株系; 3301: 转空载体拟南芥; KAN4-B1-3: 转BjuB.KAN4基因拟南芥。**表示在0.01水平差异显著。
Fig. 6. Identification of transgenic Arabidopsis plants overexpressing BjuB.KAN4
A: identification of transgenic Arabidopsis by PCR; B: relative expression of BjuB.KAN4 in transgenic Arabidopsis. WT: wild-type of Arabidopsis; KB-1-6: transgenic positive lines; 3301: Arabidopsis overexpressing empty vector; KAN4-B1-3: Arabidopsis lines overexpressing BjuB.KAN4 gene. ** Significant at P < 0.01.
