玉米ZmCIPK24-2基因在盐胁迫应答中的功能研究

李健, 王逸茹, 张凌霄, 孙明昊, 秦阳, 郑军

Functional analysis of ZmCIPK24-2 gene from maize in response to salt stress

Jian LI, Yi-Ru WANG, Ling-Xiao ZHANG, Ming-Hao SUN, Yang QIN, Jun ZHENG

表1 本研究所用的引物

Table 1 Primers used in this study

引物 Primer 正向序列 Forward sequence (5'-3') 反向序列 Reverse sequence (5'-3') ZmCIPK24-2-F/R ATGGCGGGCGCGGGCGCGGG CTAGCAGGTGGTCGTCCTCA ZmCIPK24-2-Q-F3/R3 AAGGTCCAGCGTCA GGCGTAGATTTGGCA GAPDH-F/R AGGATATCAAGAAAGCTATTAAGGC GTAGCCCCACTCGTTGTCG ZmCIPK24-2-RT-F/R TGCCACAACAAAGGAGTTTATCATA TGAGAGATCCAAACCTTGAGATAGT AtACTIN-F/R GCCAATCCGGTGCTGGTAACA CATACCAGATCCAGTTCCTCCTCCC ZmCIPK24-2-IF-F/R AGCAGGCTTTGACTTTATGGCGGGCGCGGGCGCGGG TGGGTCTAGAGACTTTCTGCAGGTGGTCGTCCTCA CBL1-nluc-F/R CACGGGGGACGAGCTCGGTACCATGGGGTGCTTCCATTCCAC ACGCGTACGAGATCTGGTCGACCGTGACGAGATCGTCGA CBL4-nluc-F/R CACGGGGGACGAGCTCGGTACCATGGGCTGCGCGACGTCCAA ACGCGTACGAGATCTGGTCGACGTCACTGGCTTCTGAAC CBL8-nluc-F/R CACGGGGGACGAGCTCGGTACCATGGGGTGTGTGTCCTCCAA ACGCGTACGAGATCTGGTCGACCAACTCGTCGTCACTGG CBL9-nluc-F/R CACGGGGGACGAGCTCGGTACCATGGGGTGCTTCCATTCCAC ACGCGTACGAGATCTGGTCGACCGTGACGAGATCGTCGA CIPK24-2-cluc-F/R TACGCGTCCCGGGGCGGTACCATGGCGGGCGCGGGCGCGGG TCCTTGTAGTCCATTTGTTGGCAGGTGGTCGTCCTCA