棉花GhPIF4调控高温下花药败育机制初探

陈淼, 谢赛, 王超智, 李焱龙, 张献龙, 闵玲

Mechanism of GhPIF4 regulating anther abortion under high temperature stress in cotton

Miao CHEN, Sai XIE, Chao-Zhi WANG, Yan-Long LI, Xian-Long ZHANG, Ling MIN

表1 实验中用到的载体和菌株

Table 1 Vectors and strains used in the experiment

载体/菌株 Vector/strain 用途 Purpose pGEM-T Easy载体 pGEM-T Easy vector TA克隆 TA clone pDONOR221载体 pDONOR221 vector BP反应 BP reaction pGBW409载体 pGBW409 vector 35S:GhPIF4:His载体的构建 Construction of 35S:GhPIF4:His vector pMDC43载体 pMDC43 vector GhPIF4亚细胞定位 Subcelluar localization of GhPIF4 pGWB433载体 pGWB433 vector GhPIF4启动子表达模式分析 Analysis of GhPIF4 promoter expression pattern 大肠杆菌TOP10 E. coli TOP10 载体转化 Vector transformation 农杆菌GV3101 Agrobacterium GV3101 棉花下胚轴的转化及烟草的侵染 Transformation of cotton hypocotyl and tobacco infection