棉花GhPIF4调控高温下花药败育机制初探

陈淼, 谢赛, 王超智, 李焱龙, 张献龙, 闵玲

Mechanism of GhPIF4 regulating anther abortion under high temperature stress in cotton

Miao CHEN, Sai XIE, Chao-Zhi WANG, Yan-Long LI, Xian-Long ZHANG, Ling MIN

表2 本实验中所用的引物及用途

Table 2 Sequence and purpose of the primers used in this study

引物名称Primer name 引物序列Primer sequence (5°-3°) 用途Purpose GhPIF4-CDS-F ATGGATCACCAACATGAACAACA Gene amplification GhPIF4-CDS-R TCAGTTAAATCCCGGATTGGCAG Gene amplification GhPIF4-CDS-BP-F GGGGACAAGTTTGTACAAAAAAGCAGGCTGGATGGAT CACCAACATGAACAACA BP reaction GhPIF4-CDS-BP-R GGGGACCACTTTGTACAAGAAAGCTGGGTGTCAGTTA AATCCCGGATTGGCAG BP reaction proGhPIF4-F TTTTTGTCTCCCATTACAGTATC Gene promoter amplification proGhPIF4-R GGGTCATAAACTGGAAATTCAGA Gene promoter amplification GhUB7-qRT-F CTTGACCTTCTTCTTCTTGTGCTTG qRT-PCR GhUB7-qRT-R GAAGGCATTCCACCTGACCAAC qRT-PCR GhPIF4-CDS-qRT-F AACTACACCTCAAAGTCCCACGG qRT-PCR GhPIF4-CDS-qRT-R CCCGGATTGGCAGTGGTC qRT-PCR GhTAA1-qRT-F GGTCTTAAAAAGGTTGGGGCTTA qRT-PCR GhTAA1-qRT-R TTAGCTTGGGTATGTGTTTGATTTG qRT-PCR GhYUC2-qRT-F ACCGATGTGGGTTTTGGCGAAT qRT-PCR GhYUC2-qRT-R CTCAGCATTTTCCCCGGTAGCA qRT-PCR GhCYP71A13-qRT-F CGTAAACAGACCGAAACGCAGC qRT-PCR GhCYP71A13-qRT-R GTGGTTGCGGAAAAGAGTTCGC qRT-PCR