), 谢赛, 王超智, 李焱龙, 张献龙, 闵玲*()
), Sai XIE, Chao-Zhi WANG, Yan-Long LI, Xian-Long ZHANG, Ling MIN*()
图3. GhPIF4 组织表达及高温诱导表达分析
A: GhPIF4在‘H05’中的组织表达分析。B: GhPIF4在‘YZ1’中的组织表达分析。C: proGhPIF4:GUS转基因棉花组织表达情况; a: 开花当天的整体花药; b: 开花当天的单个花药; c: 萼片; d: 花瓣; e: 幼嫩的叶子。D: GhPIF4在‘H05’不同发育时期的花药中高温诱导表达分析。a、c、d、e图, bar = 1 cm; b图bar = 100 μm; HN和HH分别表示正常温度和高温胁迫条件下的‘H05’; < 7 mm表示花蕾长度小于7 mm花药的混合; **P < 0.01。
Fig. 3. Expression of GhPIF4 in different tissue and induced by high temperature in Gossypium hirsutum
A and B: the expression analysis of GhPIF4 in H05 (A) and YZ1 (B), respectively. C: the expression pattern of proGhPIF4:GUS transgenic cotton; a: whole anther from the blooming flower; b: single anther from the blooming flower; c: sepal; d: petal; e: young leaf. D: the heat-induced expression analysis of GhPIF4 in ‘H05’ anthers at different development stages. Figures a, c, d, e, bar = 1 cm; figure b, bar = 100 μm; HN and HH refer to ‘H05’ under normal temperature and high temperature stress conditions, respectively; < 7 mm represents a mixture of flower buds less than 7 mm in length; **P < 0.01.
