基于侯选基因标记的四倍体马铃薯休眠QTL关联分析

李竟才, 王强林, 宋威武, 黄维, 肖桂林, 吴承金, 顾钦, 宋波涛

Association analysis of dormancy QTL in tetraploid potato via candidate gene markers

Jing-Cai LI, Qiang-Lin WANG, Wei-Wu SONG, Wei HUANG, Gui-Lin XIAO, Cheng-Jin WU, Qin GU, Bo-Tao SONG

表2 候选基因标记引物

Table 2 Marker primers for candidate genes

引物名称a Primer name a 染色体 Chr. 连锁QTL Linked QTL 上游引物 Forward primer (5°-3°) 下游引物 Reverse primer (5°-3°) 退火温度 Annealing temperature (℃) S199 Chr05 DorB5.3 TGCCTACTGCCCAAAACATT ACTGGCTGGGAAGCATACAC 55 GWD Chr05 DorB5.3 TCCATCCTGAGACTGGAGATAC ACTTGTACTGCAGGACTGGAAG 60 G6pt Chr05 DorB5.3 GGCTCACACAATTGGTCATGTG CCAAGATTGCAATAGCAGCACC 60 s1939 Chr05 DorB5.3 TGAGATACTTTGTGTGCTCC AAATTGGTTTTCCAGATTGA 56 STI058 Chr05 DorB5.3 CAAGCACGTTACAACAAGCAA TTGAAGCATCACATACACAAACA 60-54 FK Chr06 DorB6.3 GCTTTGGCGTTCGTGACTCTAC AGTGGTGTCAACAGTCTTCACG 60 S1711 Chr06 DorE6.17 TTCTTCAGGGTCCTCTTTCGG AGTGCTTCCTCGCATGGGATT 67 S1614 Chr06 DorE6.17, DorE6.19 TCGTGGGTCAAGGTTGTTCAT ATGGTGGATTAGACCTAGTTGCTG 65 Dpe-P Chr04 DorE4.6 CACTACTTTTCAATCTCCTATCCC GCATAGTCACGAACTTTTTTCC 56 α-Glu Chr04 DorE4.6 ACCAAGCTGTGGTTAACCAGAG GCAGTTGCGAATAACTGTGGCA 60 Ppe Chr03 DorB3.17 TCCGTCCATCCTTTCTGCTAAC AACTCCACCATCAACTTCAATC 57