脯氨酸羟化酶GhP4H2在棉花纤维发育中的功能研究

高璐, 许文亮

GhP4H2 encoding a prolyl-4-hydroxylase is involved in regulating cotton fiber development

GAO Lu, XU Wen-Liang

表1 本研究所用引物

Table 1 Primers used in this study

引物 Primer 正向序列 Forward sequence (5'-3') 反向序列 Reverse sequence (5'-3') Gh_D12G1409RT TAGAACCCAAGCTGGTCAGG TCGATTTAGACCCTGTCGATG Gh_D01G1917RT AGATCTCATGCGCCGTCA AGGCAAAGAAAGAAACAAGTGTG Gh_D07G2484RT TTTGCCTTTTTCTGTGAGCA GCTGGTTGGGATGAACACTT Gh_D10G0598RT CTATGAGTGTGATTGGCGTACAGGTAA AATCCGTGGTCACGTTATATTTGG Gh_D08G2475RT TAACCCACCAAGTGGGAAAC TGAGCTTAAGGGTGGTGCAT Gh_A07G0433RT TGAAGAAGACGAAGAACCATCACCA AGAGCCAGAGCTTTCAACTGATGT Gh_D05G0131RT TTAAGCCACCCATTGACCTC TCCTGAAAGAACCGGACAAC Gh_D12G2178RT TCTTTTCCCTGGTGAGTGCT AACGGCAGCTTCAAGATCAG GhGalT1RT TCCCTCCTCACCTTCGCCATTG CCTTGACGATCAGAAGGCATCCAC GhGalT4RT GATGATACTTTGAAAATCGTTGCT ATTTTCTTCAAAGTTTCACCGCT GhGalT6RT TCAGGAGTATGTACCCCAGCTTGC TGCAAATGAAGTCACATGTCGAC GhGalT7RT CCTCACCAGATCAACAGCCCTCT CCGTTGAAGGCCCTGATGATC GhP4H2RT ATCGGAATGTGCAAAGAAGG AGCCAGGAAGTTCTGCAGTT GhUBI1RT CTGAATCTTCGCTTTCACGTTATC GGGATGCAAATCCTTCGTGAAAAC GhP4H2 RNAiL1 CTTGGATCCGTGCAAAGAAGGGAATTG GGGTCTAGAAATAGCCAGGAAGTTCTG GhP4H2RNAiL2 GGGGAGCTCGTGCAAAGAAGGGAATTG CTTGCGGCCGCAATAGCCAGGAAGTTCT GhP4H2OE GGGTCTAGAATGGCTATTGAGAGGATTT CTTGTCGACTCAACATACTTTGCAGCTT