78份四川小麦育成品种(系)条锈病抗性鉴定与抗条锈病基因分子检测

习玲, 王昱琦, 朱微, 王益, 陈国跃, 蒲宗君, 周永红, 康厚扬

Identification of resistance to wheat and molecular detection of resistance genes to wheat stripe rust of 78 wheat cultivars (lines) in Sichuan province

XI Ling, WANG Yu-Qi, ZHU Wei, WANG Yi, CHEN Guo-Yue, PU Zong-Jun, ZHOU Yong-Hong, KANG Hou-Yang

表1 抗条锈病基因的分子标记及引物序列

Table 1 Molecular markers and primer sequences of wheat stripe rust resistance genes

Yr基因 Yr gene 类别a Type a 分子标记 Molecular marker 引物序列 Primer sequence (5′?3′) 参考文献 Reference QYr.nwafu-4BL KASP AX-110963704 GAAGGTGACCAAGTTCATGCTTCGTTTGCCTATGGTTCAAAAC [31] GAAGGTCGGAGTCAACGGATTTCGTTTGCCTATGGTTCAAAAG TCTCCATGACACGGGAGGAT Yr5 DM Yr5_B GGGAACACTTCACGATCA [32] AATTCCTTCATGCCTTCC DM Yr5_A CGCTTAATTCCCCTTCCTTC [32] TGGCTCCTTATTCGTTCTCTTTC KASP Yr5F GAAGGTGACCAAGTTCATGCTGCGCCCCTTTTCGAAAAAATA [32] Yr5H GAAGGTCGGAGTCAACGGATTCTAGCATCAAACAAGCTAAATA Yr5R ATGTCGAAATATTGCATAACATGG Yr10 AFLP SC200 CTGCAGAGTGACATCATACA [33] TCGAACTAGTAGATGCTGGC DM Yr10-5 GGAAATGTGGCGGAGTACCA [34] CGGAAGGGAGAACCACTGTC DM Yr10-6 CAGCTTGACAAGGGCGAGTA [34] CGCTTGTCGCCAATTCCAAA Yr15 DM Y15K1_F2 GGAGATAGAGCACATTACAGAC [35] uhw301R GGAGATAGAGCACATTACAGAC DM WJL2F CCTTGTGTGCTACCAGGGTT [36] WJL2R GGAACTCAAGCCCTTCTGCT DM WJL3F AAAAGAGCTCGCCTCCTACG [37] WJL3R GCCATGATGAGATCGGGAGG Yr17 SCAR SC2372 AGGGGCTACTGACCAAGGCT [38] TGCAGCTACAGCAGTATGTACACAAAA Yr18 STS csLV34 GTTGGTTAAGACTGGTGATGG [39] TGCTTGCTATTGCTGAATAGT Cssfr1 L34DINT9F TTGATGAAACCAGTTTTTTTTCTA [39] L34PLUSR GCCATTTAACATAATCATGATGGA KASP Lr34-KASP-E11 GAAGGTGACCAAGTTCATGCTGGGAGCATTATTTTTTTCCATCA [40] GAAGGTCGGAGTCAACGGATTGGGAGCATTATTTTTTTCCATCT AGCGAATCCAGTATGGAAAT Yr26 STS Xwe173 GGGACAAGGGGAGTTGAAGC [41] GAGAGTTCCAAGCAGAACAC STS Xwe33 TAAACCAAGTCCCCCAAA [41] GGAGTCCATCTTCACCGA Yr28 InDel ZCZp343F TGTGTCATGTTTGGTCGATAGG [42] ZCZp342R TCCTCCCTTGTAGCTTCACG