棉花CRISPR/Cas9基因编辑有效sgRNA高效筛选体系的研究

周冠彤, 雷建峰, 代培红, 刘超, 李月, 刘晓东

Efficient screening system of effective sgRNA for cotton CRISPR/Cas9 gene editing

ZHOU Guan-Tong, LEI Jian-Feng, DAI Pei-Hong, LIU Chao, LI Yue, LIU Xiao-Dong

表1 本研究中使用的引物序列

Table 1 Primer sequences used in this study

引物名称 Primer name 上游引物序列 Forward sequence (5'-3') 下游引物序列 Reverse sequence (5'-3') GhU6-5P-MAPKKK2-sg AAGGGTTCCCAGCTGACATA TATGTCAGCTGGGAACCCTT GhU6-5P-AE-sg GAGTTTGGAGGGCTTACAAT ATTGTAAGCCCTCCAAACTC GhU6-5P-2-PDS-sg GAAGCGAGAGATGTTCTAGG CCTAGAACATCTCTCGCTTC GhU6-5P-2-CLA1-sg TATGCTCGCGGAATGATCAG CTGATCATTCCGCGAGCATA Test MAPKKK2-sg CCATGTCGTAGCTTATAAAGG TCATTTACCTTCTCTTCCCAG Test AE-sg AGACTTGTTTCAATGGACTC AATAAGCTGACAGCAGTTGG Test PDS-sg TGCATGATCCATCACTCAAGTTT GAACGAAAGGCCCTTTCTTTC Test CLA1-sg GGATCTGAAAGGTGAAAGGAATC TACCGTGATACTTGTCAGCAGCT