棉花GbSTK基因调控开花和黄萎病抗性的功能研究

崔静, 王志城, 张新雨, 柯会锋, 吴立强, 王省芬, 张桂寅, 马峙英, 张艳

Function study of cotton GbSTK gene in regulating flowering and Verticillium wilt resistance

CUI Jing, WANG Zhi-Cheng, ZHANG Xin-Yu, KE Hui-Feng, WU Li-Qiang, WANG Xing-Fen, ZHANG Gui-Yin, MA Zhi-Ying, ZHANG Yan

图3 过表达GbSTK拟南芥表型鉴定及开花标志基因的表达分析 a~c: 过表达GbSTK基因促进拟南芥提早开花; d: 开花时转基因植株与对照植株莲座叶片数比较; e~g: 转基因植株与对照植株中SOC1、FT和LFY基因的表达。误差线表示标准差, *, ***分别表示在0.05和0.001水平显著差异。WT: 野生型拟南芥; OE: 转GbSTK基因拟南芥; OE1和OE2: 转GbSTK基因拟南芥株系。

Fig. 3 Phenotypic evaluation of overexpression of GbSTK in Arabidopsis and expression analysis of the flowering marker genes a-c: overexpression (OE) of GbSTK in Arabidopsis led to early flowering; d: the transgenic Arabidopsis showed significantly fewer rosette leaves when flowering; e-g: the transgenic Arabidopsis showed significantly higher expression of SOC1 and FT, rather than LFY. Error bars indicate standard error (SE). *, *** indicate significant difference at the 0.05 and 0.001 probability levels, respectively. WT: wild type Arabidopsis; OE: GbSTK transgenic Arabidopsis; OE1 and OE2: different GbSTK transgenic Arabidopsis lines.