棉花GbSTK基因调控开花和黄萎病抗性的功能研究

崔静, 王志城, 张新雨, 柯会锋, 吴立强, 王省芬, 张桂寅, 马峙英, 张艳

Function study of cotton GbSTK gene in regulating flowering and Verticillium wilt resistance

CUI Jing, WANG Zhi-Cheng, ZHANG Xin-Yu, KE Hui-Feng, WU Li-Qiang, WANG Xing-Fen, ZHANG Gui-Yin, MA Zhi-Ying, ZHANG Yan

图4 诱饵载体pGBKT7-GbSTK的构建与毒性检测 M: DNA marker DL5000; 1~2: GbSTK-T双酶切; 3~6: pGBKT7双酶切; 7: pGBKT7-GbSTK阳性克隆。d: 诱饵载体pGBKT7- GbSTK毒性检测。

Fig. 4 Construction of bait vector of pGBKT7-GbSTK and identification of toxicity M: DNA marker DL5000; 1-2: double digestion of GbSTK-T vector; 3-6: double digestion of pGBKT7 vector; 7: screening pGBKT7- GbSTK positive clones. d: detection of toxicity for the bait vector of pGBKT7-GbSTK.