水稻矮化宽叶突变体osdwl1的生理特性和基因定位

黄妍, 贺焕焕, 谢之耀, 李丹莹, 赵超越, 吴鑫, 黄福灯, 程方民, 潘刚

Physiological characters and gene mapping of a dwarf and wide-leaf mutant osdwl1 in rice (Oryza sativa L.)

HUANG Yan, HE Huan-Huan, XIE Zhi-Yao, LI Dan-Ying, ZHAO Chao-Yue, WU Xin, HUANG Fu-Deng, CHENG Fang-Min, PAN Gang

图2 突变体osdwl1及其对照叶片的表型特征 A: 突变体及其对照幼叶及剑叶叶宽; B: 突变体及其对照剑叶石蜡切片, 蓝色三角形指示的是小维管束, LV代表大维管束; C: 突变体及其对照整个剑叶的小维管束数, SV代表小维管束; D: 突变体及其对照剑叶的小维管束之间的间距; E, F: 维管束之间的上表皮细胞数(E)及其宽度(F); G: 突变体及其对照剑叶上下表皮的扫描电镜观察, 双向箭头显示两个SV之间距; H, I: 突变体及其对照剑叶下表皮(H)和上表皮(I)的刺毛数。标尺为200 μm。* 表示在0.05水平上差异显著; ** 表示在0.01水平上差异显著(t-test)。

Fig. 2 Phenotypic characteristics of leaf blade in osdwl1 and its wild-type plants A: leaf blade width of the osdwl1 and its WT plants; B: transverse cross-sections of the flag leaf blade of the osdwl1 and its WT plants, blue triangles indicate small vascular bundles (SV), and LV means large vascular bundle; C: number of SVs in the whole flag leaves of osdwl1 and its WT plants; D: the distance between the two SVs in the osdwl1 and its WT plants; E: adaxial epidermal cell numbers between two SVs; F: adaxial epidermal cell width; G: SEM analysis of the abaxial and adaxial epidermis of the flag leaf blade in the osdwl1 and its WT plants, double arrow means the distance between two SVs; H, I: number of macrohairs and microhairs on the abaxial (H) and adaxial epidermis (I) of the flag leaf in the osdwl1 and its wild-type plants. Bar = 200 μm. Values marked with * and ** indicate significant differences by Student’s t-test at P < 0.05 and P < 0.01, respectively.