甘蔗ScCRT1基因克隆及其应答SCMV侵染分子机制的研究

张海, 程光远, 杨宗桃, 王彤, 刘淑娴, 商贺阳, 赵贺, 徐景升

Cloning of sugarcane ScCRT1 gene and its response to SCMV infection

ZHANG Hai, CHENG Guang-Yuan, YANG Zong-Tao, WANG Tong, LIU Shu-Xian, SHANG He-Yang, ZHAO He, XU Jing-Sheng

图1 甘蔗钙网蛋白(ScCRT1)氨基酸序列比对与结构域 高粱: SbCRT1 (XP_021307591.1); 玉米: ZmCRT1 (XP_008670080.1); 谷子: SiCRT1 (XP_004981159.1); 二穗短柄草: BdCRT1 (XP_003563166.1); 水稻: OsCRT1 (XP_015628738.1); 拟南芥: AtCRT1 (NP_176030.1)。黑色背景中的蛋白残基为相同的部分。箭头表示3个结构域(N, P和C)的起始位置。预测的信号肽、保守的CRT家族基序(CRT motif 1: KLDCGGGYVKLL; CRT motif 2: IMFGPDICG)、3个重复的残基(A: PXXIXDPXX KKPEXWDD; B: GXWXAXXIXNPXYK, X为任意氨基酸)[1]和内质网滞留信号HDEL用黑色下划线标记。假定的核靶向序列(PPKKIKDPE)用红色下画线标记。

Fig. 1 Amino acid sequence alignment and domains of Saccharum calreticulin (ScCRT1) Sorghum bicolor: SbCRT1 (XP_021307591.1); Zea mays: ZmCRT1 (XP_008670080.1); Setaria italic: SiCRT1 (XP_004981159.1); Brachypodium distachyon: BdCRT1 (XP_003563166.1); Oryza sativa: OsCRT1 (XP_015628738.1); Arabidopsis thaliana: AtCRT1 (NP_176030.1). Protein residues on the black background in all of these proteins are identical. The arrows indicate the approximate positions of the three domains (N, P and C). The predicted signal peptide, conserved CRT family motifs (CRT motif 1: KHEQKLDCGGGYVKLL; CRT motif 2: IMFGPDICG), triplicate repeats (A: PXXIXDPXX KKPEXWDD; B: GXWXAXXIXNPXYK, X represents arbitrary residue) [1] and the endoplasmic reticulum retention sequence HDEL was underlined in black. The putative nuclear targeting sequence (PPKKIKDPE) was underlined in red.