甘蔗ScCRT1基因克隆及其应答SCMV侵染分子机制的研究

张海, 程光远, 杨宗桃, 王彤, 刘淑娴, 商贺阳, 赵贺, 徐景升

Cloning of sugarcane ScCRT1 gene and its response to SCMV infection

ZHANG Hai, CHENG Guang-Yuan, YANG Zong-Tao, WANG Tong, LIU Shu-Xian, SHANG He-Yang, ZHAO He, XU Jing-Sheng

图6 Y2H检测ScCRT1与SCMV-6K2的互作 pNubG-Fe65和pTSU2-APP组合作为阳性对照, pNubG-Fe65和pPR3-N组合作为阴性对照。DDO+X-Gal: 添加了5-溴-4-氯-3-吲哚-β-D-半乳糖苷的缺少亮氨酸(Leu)和色氨酸(Trp)的酵母合成限定基本培养基; QDO+X-Gal: 添加了X-Gal的缺少亮氨酸(Leu)、色氨酸(Trp)、组氨酸(His)和腺嘌呤(Ade)的酵母合成限定基本培养基。

Fig. 6 Protein-protein interactions between ScCRT1 and SCMV-6K2 by Y2H assay The positive and negative controls are yeast cotransformants with plus pNubG-Fe65 pTSU2-APP and pNubG-Fe65 plus pPR3-N, respectively. DDO+X-Gal: synthetic defined yeast minimal medium lacking Leu and Trp but plus the 5-Bromo-4-Chloro-3-Indolyl β-D-Galactopyranoside; QDO+X-Gal: synthetic defined yeast minimal medium lacking Leu, Trp, His, and Ade but plus the X-Gal.