甘蔗ScCRT1基因克隆及其应答SCMV侵染分子机制的研究

张海, 程光远, 杨宗桃, 王彤, 刘淑娴, 商贺阳, 赵贺, 徐景升

Cloning of sugarcane ScCRT1 gene and its response to SCMV infection

ZHANG Hai, CHENG Guang-Yuan, YANG Zong-Tao, WANG Tong, LIU Shu-Xian, SHANG He-Yang, ZHAO He, XU Jing-Sheng

图7 BiFC检测ScCRT1与SCMV-6K2的互作 ScCRT1融合于YFP的C末端, SCMV-6K2融合于YFP的N末端, 在本氏烟叶片中瞬时表达, 48 h后激光共聚焦观察(标尺为25 μm)。

Fig. 7 Interactions between ScCRT1 and SCMV-6K2 by BiFC assay ScCRT1 was fused to the C-terminal half of YFP, while SCMV-6K2 was fused to the N-terminal half of YFP. ScCRT1-YC and YN- SCMV-6K2 were transiently co-expressed in the epidermal cells of N. benthamiana. The fluorescent signal was monitored by confocal microscopy at 48 hours post inoculation (Bar = 25 μm).