棉花花器官突变体的鉴定及候选基因的克隆
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杨琴莉 1( ), 杨多凤 1, 丁林云 1, 赵汀 2, 张军 2, 梅欢 2, 黄楚珺 2, 高阳 1, 叶莉 1, 高梦涛 1, 严孙艺 2, 张天真 1,2, 胡艳 1,2,*( )
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Identification of a cotton flower organ mutant 182-9 and cloning of candidate genes
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YANG Qin-Li 1( ), YANG Duo-Feng 1, DING Lin-Yun 1, ZHANG Ting 2, ZHANG Jun 2, MEI Huan 2, HUANG Chu-Jun 2, GAO Yang 1, YE Li 1, GAO Meng-Tao 1, YAN Sun-Yi 2, ZHANG Tian-Zhen 1,2, HU Yan 1,2,*( )
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图3. PCR检测转基因材料(182-9) NPTII基因 M: DNA分子量Marker; W0: 阴性对照; 1: 转基因株系182-9; 2: 转基因株系182-36; 3: 转基因株系182-91; 4: 转基因株系182-150; 5: 转基因株系182-172; 6: 转基因株系182-173; 7: 转基因株系182-187。
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Fig. 3. PCR detection of NPTII gene in transgenic mutant 182-9 M: DNA molecular-weight markers; W0: negative control; 1: transgenic line 182-9; 2: transgenic line 182-36; 3: transgenic line 182-91; 4: transgenic line 182-150; 5: transgenic line 182-172; 6: transgenic line 182-173; 7: transgenic line 182-187.
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