棉花花器官突变体的鉴定及候选基因的克隆
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杨琴莉 1( ![](http://zwxb.chinacrops.org/images/email.png) ), 杨多凤 1, 丁林云 1, 赵汀 2, 张军 2, 梅欢 2, 黄楚珺 2, 高阳 1, 叶莉 1, 高梦涛 1, 严孙艺 2, 张天真 1,2, 胡艳 1,2,*( ![](http://zwxb.chinacrops.org/images/email.png) )
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Identification of a cotton flower organ mutant 182-9 and cloning of candidate genes
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YANG Qin-Li 1( ![](http://zwxb.chinacrops.org/images/email.png) ), YANG Duo-Feng 1, DING Lin-Yun 1, ZHANG Ting 2, ZHANG Jun 2, MEI Huan 2, HUANG Chu-Jun 2, GAO Yang 1, YE Li 1, GAO Meng-Tao 1, YAN Sun-Yi 2, ZHANG Tian-Zhen 1,2, HU Yan 1,2,*( ![](http://zwxb.chinacrops.org/images/email.png) )
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图4. Southern杂交检测突变体182-9中外源基因NPTII的整合 M: DNA marker; 1: 转基因受体W0; C: 质粒; 2: 转基因株系182-187; 3: 转基因株系182-36; 4: 转基因株系182-173; 5: 转基因株系182-9 (箭头所示)。
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Fig. 4. Southern blotting of NPTII gene in mutant 182-9 genome M: DNA marker; 1: transgenic receptor W0; C: plasmid; 2: transgenic line 182-187; 3: transgenic line 182-36; 4: transgenic line 182-173; 5: transgenic line 182-9 (indicated by the arrow).
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![](http://zwxb.chinacrops.org/fileup/0496-3490/FIGURE/2021-47-10/Images/0496-3490-47-10-1854/img_4.png) |
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