棉花花器官突变体的鉴定及候选基因的克隆
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杨琴莉 1( ![](http://zwxb.chinacrops.org/images/email.png) ), 杨多凤 1, 丁林云 1, 赵汀 2, 张军 2, 梅欢 2, 黄楚珺 2, 高阳 1, 叶莉 1, 高梦涛 1, 严孙艺 2, 张天真 1,2, 胡艳 1,2,*( ![](http://zwxb.chinacrops.org/images/email.png) )
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Identification of a cotton flower organ mutant 182-9 and cloning of candidate genes
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YANG Qin-Li 1( ![](http://zwxb.chinacrops.org/images/email.png) ), YANG Duo-Feng 1, DING Lin-Yun 1, ZHANG Ting 2, ZHANG Jun 2, MEI Huan 2, HUANG Chu-Jun 2, GAO Yang 1, YE Li 1, GAO Meng-Tao 1, YAN Sun-Yi 2, ZHANG Tian-Zhen 1,2, HU Yan 1,2,*( ![](http://zwxb.chinacrops.org/images/email.png) )
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图5. PCR检测外源T-DNA在基因组中插入位点 A: PCR扩增条带; B: 扩增序列的测序结果(红色部分为棉花基因组序列, 其他为T-DNA序列)。M: DNA marker; W0: 转基因受体; 182-9: 转基因株系。
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Fig. 5. PCR detection of the T-DNA insertion site in mutant 182-9 A: diagram of PCR detection; B: the sequencing results of the amplified sequence from 182-9 (the red part is the cotton genome sequence, and the others are T-DNA sequences). M: DNA marker; W0: transgenic receptor; 182-9: transgenic line.
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![](http://zwxb.chinacrops.org/fileup/0496-3490/FIGURE/2021-47-10/Images/0496-3490-47-10-1854/img_5.png) |
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