棉花花器官突变体的鉴定及候选基因的克隆
杨琴莉1(
), 杨多凤1, 丁林云1, 赵汀2, 张军2, 梅欢2, 黄楚珺2, 高阳1, 叶莉1, 高梦涛1, 严孙艺2, 张天真1,2, 胡艳1,2,*()
), 杨多凤1, 丁林云1, 赵汀2, 张军2, 梅欢2, 黄楚珺2, 高阳1, 叶莉1, 高梦涛1, 严孙艺2, 张天真1,2, 胡艳1,2,*()
Identification of a cotton flower organ mutant 182-9 and cloning of candidate genes
YANG Qin-Li1(), YANG Duo-Feng1, DING Lin-Yun1, ZHANG Ting2, ZHANG Jun2, MEI Huan2, HUANG Chu-Jun2, GAO Yang1, YE Li1, GAO Meng-Tao1, YAN Sun-Yi2, ZHANG Tian-Zhen1,2, HU Yan1,2,*()
), YANG Duo-Feng1, DING Lin-Yun1, ZHANG Ting2, ZHANG Jun2, MEI Huan2, HUANG Chu-Jun2, GAO Yang1, YE Li1, GAO Meng-Tao1, YAN Sun-Yi2, ZHANG Tian-Zhen1,2, HU Yan1,2,*()
图5. PCR检测外源T-DNA在基因组中插入位点
A: PCR扩增条带; B: 扩增序列的测序结果(红色部分为棉花基因组序列, 其他为T-DNA序列)。M: DNA marker; W0: 转基因受体; 182-9: 转基因株系。
Fig. 5. PCR detection of the T-DNA insertion site in mutant 182-9
A: diagram of PCR detection; B: the sequencing results of the amplified sequence from 182-9 (the red part is the cotton genome sequence, and the others are T-DNA sequences). M: DNA marker; W0: transgenic receptor; 182-9: transgenic line.
