水稻苗期根部性状的遗传分析和最长根长QTL qLRL4的精细定位

田彪, 丁仕林, 刘朝雷, 阮班普, 姜洪真, 郭锐, 董国军, 胡光莲, 郭龙彪, 钱前, 高振宇

Genetic analysis of seedling root traits and fine mapping of the QTL qLRL4 for the longest root length in rice

TIAN Biao, DING Shi-Lin, LIU Chao-Lei, RUAN Ban-Pu, JIANG Hong-Zhen, GUO Rui, DONG Guo-Jun, HU Guang-Lian, GUO Long-Biao, QIAN Qian, GAO Zhen-Yu

表2 候选基因实时定量PCR引物

Table 2 Primers of candidate genes and reference for qRT-PCR

基因 Gene 正向序列 Forward primer (5′-3′) 反向序列 Reverse primer (5′-3′) LOC_Os04g58870 AATCACTTGAGCAAGCTCTTGG ATGCAAACATGCACAATGCAG LOC_Os04g58880 AAGAGCTGCGGTCAAAGAGAG ACTCACGCAACTCCGTATCTGG LOC_Os04g58890 TCGTCGCCGACAGGGTG AGGTCGGTCCCCTCCTCG LOC_Os04g58900 ACGTATGATTTCCCTGTTGATGTG ACCTCATCTTCCTTCCCAGTAAATC LOC_Os04g58910 AGAAATGGAAGCCTGTGAACGAC TCATGCTGGCCTCTGCGTC LOC_Os04g58920 AGCTGTGCGTGGTGGCGC CTGCAAGCAGGGCGAGTTCC LOC_Os04g58940 AGGCTCTTCGGCTACCTGC ATCAGCTCATCCCCATCACC LOC_Os04g58960 AGCAGATGACCAATGGTGGG TGGTTGCTGGGTGAGCTAGAAC Histone H3 GGTCAACTTGTTGATTCCCCTCT AACCGCAAAATCCAAAGAACG