玉米籽粒缺陷突变基因dek54的精细定位及候选基因分析

周练, 刘朝显, 陈秋栏, 王文琴, 姚顺, 赵子堃, 朱思颖, 洪祥德, 熊雨涵, 蔡一林

Fine mapping and candidate gene analysis of maize defective kernel mutant dek54

ZHOU Lian, LIU Chao-Xian, CHEN Qiu-Lan, WANG Wen-Qin, YAO Shun, ZHAO Zi-Kun, ZHU Si-Ying, HONG Xiang-De, XIONG Yu-Han, CAI Yi-Lin

表1 dek54定位SSR标记引物序列

Table 1 Sequences of SSR markers for dek54 mapping

标记类型 Type 标记名称 Name 正向引物序列 Forward sequence (5′-3′) 反向引物序列 Reverse sequence (5′-3′) SSR SSR23403 ACTAGTATGTGGATTGCTCGTCG GCTGCTGAGCTGTATGTACCA SSR SSR1 CCCTGACGAAAGCATGAATGAG ACAGATGACTCTGCACCTCAAG SSR SSR2 TGCCCATAAGAGCTTCGAGGATA AGCCTTAGTTGTCAGTCCATCG SSR SSR3 TGCCCATAAGAGCTTCGAGGATA AGCCTTAGTTGTCAGTCCATCG SSR SSR4 CCATCTGTACTAATGGCACCTGA GCCAGCAAAGCTTTTCAAGAGT SSR SSR5 GGCTACATAAGATGCAAAGCGG TACCCTTTGACAGAGCCTACCT SSR SSR6 GGTGCCAAAAACATCTCCCAAC TAGCGTGGGGTCATAGCAACA SSR SSR7 CATGGCCAAAATATCGCACGAG TGACGTACATGAACACCTCGG SSR SSR8 GCTAGGTGCAGTGTCTCTGCTT CCTTGAACGTGGGGTAGGCT SSR SSR10308 GCAAATGTTCTGTGCAAGGCTA GCCCCACAAGAACTCCATCTAT