玉米籽粒缺陷突变基因dek54的精细定位及候选基因分析
周练(
), 刘朝显, 陈秋栏, 王文琴, 姚顺, 赵子堃, 朱思颖, 洪祥德, 熊雨涵, 蔡一林*()
), 刘朝显, 陈秋栏, 王文琴, 姚顺, 赵子堃, 朱思颖, 洪祥德, 熊雨涵, 蔡一林*()
Fine mapping and candidate gene analysis of maize defective kernel mutant dek54
ZHOU Lian(), LIU Chao-Xian, CHEN Qiu-Lan, WANG Wen-Qin, YAO Shun, ZHAO Zi-Kun, ZHU Si-Ying, HONG Xiang-De, XIONG Yu-Han, CAI Yi-Lin*()
), LIU Chao-Xian, CHEN Qiu-Lan, WANG Wen-Qin, YAO Shun, ZHAO Zi-Kun, ZHU Si-Ying, HONG Xiang-De, XIONG Yu-Han, CAI Yi-Lin*()
图4. dek54的连锁SSR标记筛选和精细定位
A: umc2160的PCR扩增产物在WP和MP之间具有多态性; B: 通过33个dek54单株的基因分型确定SSR连锁标记umc2160; C: 通过F2群体1566个单株对dek54进行精细定位。dek54定位标记SSR6和SSR7之间物理距离约为290 kb的区间内。黑色垂直实线上方为分子标记名称, 下方数字为该标记鉴定的交换单株数量。
Fig. 4. Linkage SSR marker screening and fine mapping of dek54
A: the polymorphic PCR products amplified by umc2160 between WP and MP; B: confirmation of linkage SSR marker umc2160 by genotyping 33 dek54 mutant plants; C: fine mapping of dek54 using F2 population including 1566 individuals. dek54 was mapped to an interval about 290 kb flanked by SSR6 and SSR7. The symbols above and below the black vertical solid lines represent the molecular marker and the number of recombinants, respectively.
