玉米籽粒缺陷突变基因<i>dek54</i>的精细定位及候选基因分析

玉米籽粒缺陷突变基因dek54的精细定位及候选基因分析

周练(

), 刘朝显, 陈秋栏, 王文琴, 姚顺, 赵子堃, 朱思颖, 洪祥德, 熊雨涵, 蔡一林^*(

)

Fine mapping and candidate gene analysis of maize defective kernel mutant dek54

ZHOU Lian(

), LIU Chao-Xian, CHEN Qiu-Lan, WANG Wen-Qin, YAO Shun, ZHAO Zi-Kun, ZHU Si-Ying, HONG Xiang-De, XIONG Yu-Han, CAI Yi-Lin^*(

)

图6. Zm00001d019294 CRISPR/Cas9靶向突变表型鉴定
A: Zm00001d019294的CRISPR/Cas9靶位点序列。sgRNA靶序列为绿色, PAM (protospacer-adjacent motif)序列为橙色, 红色字母和短线分别代表插入和缺失; B: dek54-cas9-1xMo17的F₂成熟果穗; C: 成熟的WT和dek54-cas9-1籽粒; D: 成熟的WT和dek54-cas9-1籽粒的纵切。Em: 胚; En: 胚乳。标尺为0.5 cm。

Fig. 6. Phenotypic characterization of CRISPR/Cas9 targeted mutation of Zm00001d019294
A: the sequence in the Zm00001d019294 locus targeted using CRISPR/Cas9. The sgRNA target sequence is green. Protospacer-adjacent motif (PAM) is blue. Red letters and dashes represent insertions and deletions, respectively. B: mature F₂ ear of dek54-cas9-1xMo17. C: mature WT and dek54-cas9-1 kernels. D: longitudinal paraffin sections of mature WT and dek54-cas9-1 kernels. Em: embryo; En: endosperm. Bars: 0.5 cm.