大环内酯类和高表达玉米C4-PEPC基因对水稻耐旱性的影响

宋凝曦, 李霞, 王净, 吴博晗, 曹悦, 杨杰, 谢寅峰

Effects on drought tolerance by pladienolide B and rice with high expression of C4-PEPC

SONG Ni-Xi, LI Xia, WANG Jin, WU Bo-Han, CAO Yue, YANG Jie, XIE Yin-Feng

表6 不同处理对水稻叶片ABA含量、相对含水量、PEPC以及SnRKs相关基因表达量的影响

Table 6 Changes of different treatments on ABA contents, relative water content, and the relative expression level of PEPC and SnRKs related genes in rice leaves

测定指标 Measurement index 转玉米C4-PEPC水稻 C4-PEPC rice (PC) Kitaake (WT) CK PEG PEG+PB PEG+PB+EGTA CK PEG PEG+PB PEG+PB+EGTA ABA 含量 ABA content (μg g-1) 8.16 d 12.95 a 5.63 e 9.78 b 9.43 c 6.86 b 4.92 f 8.26 d 相对含水量 Relative water content (%) 87.43 a 76.43 b 73.47 c 59.38 e 89.56 a 68.17 d 62.42 e 51.39 f C4-PEPC相对表达量 Relative expression of C4-PEPC 2.27 d 6.44 a 3.27 b 2.53 c Osppc2a相对表达量 Relative expression of Osppc2a 0.96 de 1.26 c 0.55 g 1.45 b 1.04 d 1.57 a 0.78 f 1.038 d SAPK8相对表达量 Relative expression of SAPK8 1.98 e 10.53 a 1.05 c 0.88 d 1.03 g 5.39 b 2.03 f 0.41 h SAPK9相对表达量 Relative expression of SAPK9 3.09 a 2.84 b 1.54 d 0.68 f 1.04 e 1.87 c 0.97 e 1.77 c SAPK10相对表达量 Relative expression of SAPK10 0.82 d 3.09 b 2.94 b 0.75 e 1.02 c 5.81 a 0.74 e 0.66 f OsK1a相对表达量 Relative expression of OsK1a 1.75 d 14.12 a 10.05 b 1.54 e 1.03 g 10.04 b 5.43 c 1.15 f OsK24相对表达量 Relative expression of OsK24 0.97 e 16.99 c 14.41 d 0.74 g 1.01 e 12.89 a 9.06 b 0.25 f OsK35相对表达量 Relative expression of OsK35 2.12 g 13.05 a 3.76 f 5.88 d 1.04 h 7.41 b 3.99 e 6.28 c