半夏PtPAL基因的克隆、表达与酶动力学分析

何潇, 刘兴, 辛正琦, 谢海艳, 辛余凤, 吴能表

Molecular cloning, expression, and enzyme kinetic analysis of a phenylalanine ammonia-lyase gene in Pinellia ternate

HE Xiao, LIU Xing, XIN Zheng-Qi, XIE Hai-Yan, XIN Yu-Feng, WU Neng-Biao

表1 本研究所用引物

Table 1 Primers used for this study

引物用途 Primer function 引物名称 Primer name 引物序列 Primer sequence (5′-3′) 基因克隆 Gene cloning PtPAL-F PtPAL-R CACCTAAACCTTCTTCCGCAG GATCTTACTTGTTACTGGTTCCAAT 荧光定量PCR Fluorescence quantitative PCR PtPAL-qF PtPAL-qR PtGAPDH-qF PtGAPDH-qR CCAACATCCTGGCTCTGCTCTC GTCCTGCTTCGGCTTCGTCA TCGTCTTGAGAAATCGGCGAC GTCAAAGATGCTCGACCGCT 原核表达载体构建 Construction of prokaryotic expression vector PtPAL-Hind III-F PtPAL-Xho I-R CGCAAGCTTTTATGGCGGCCAAGTCGAACGGTCT CGCCTCGAGTTACTGGTTCCAATAACCCCTT