半夏PtPAL基因的克隆、表达与酶动力学分析

何潇, 刘兴, 辛正琦, 谢海艳, 辛余凤, 吴能表

Molecular cloning, expression, and enzyme kinetic analysis of a phenylalanine ammonia-lyase gene in Pinellia ternate

HE Xiao, LIU Xing, XIN Zheng-Qi, XIE Hai-Yan, XIN Yu-Feng, WU Neng-Biao

表2 不同植物PAL酶动力学参数比较

Table 2 Comparison of PAL kinetic properties from different plants

酶 Enzyme 温度 Temperature (℃) 米氏常数 Km (mmol L-1) 最大酶促反应速率 Vmax (nkat mg-1) 催化常数 Kcat (s-1) 催化效率 Kcat/Km (s-1 M-1) 参考文献 References PtPAL 70 0.89 63.96 6.56 7.37×103 本研究 This study 37 0.80 37.67 3.75 4.69×103 EsPAL1 37 0.144 7.45×10-3 0.588 3.87×103 Taketo et al. (2008) [20] EsPAL2 37 0.152 5.77×10-3 0.457 3.18×103 BoPAL1 50 1.01 — 10.11 1.0 ×104 Hsieh et al. (2011) [21] BoPAL2 50 0.33 — 16.04 4.86×104 CdPAL 55 0.101 — 3.36 3.32×103 Hu et al. (2011) [22] RgPAL 50 1.3 — 25×103 19.20 Zhu et al. (2013) [23] RcPAL 45 7.90 — 52.31 6.62 ×103 Ma et al. (2013) [24]