半夏<i>PtPAL</i>基因的克隆、表达与酶动力学分析

半夏PtPAL基因的克隆、表达与酶动力学分析

何潇¹^,²(

), 刘兴³, 辛正琦¹^,², 谢海艳¹^,², 辛余凤¹^,², 吴能表¹^,²^,^*(

)

Molecular cloning, expression, and enzyme kinetic analysis of a phenylalanine ammonia-lyase gene in Pinellia ternate

HE Xiao¹^,²(

), LIU Xing³, XIN Zheng-Qi¹^,², XIE Hai-Yan¹^,², XIN Yu-Feng¹^,², WU Neng-Biao¹^,²^,^*(

)

图2. 不同植物PAL氨基酸序列多重比对
一致氨基酸残基用白色字体黑色背景表示, 保守氨基酸残基用黑色字体红色背景表示。保守的Ala-Ser-Gly催化三联体用表示实心三角, 其他保守活性位点用空心三角表示, 保守活性基序用红色方框表示。AtPAL: 拟南芥(NP_187645.1); VvPAL: 葡萄(RVW44079.1); LrPAL: 百合(ASV46344.1); GbPAL: 银杏(ABU49842.1); EaPAL: 问荆(AAW80639.1); PaPAL: 背苔(AIU99853.1)。

Fig. 2. Multiple alignment of amino acid sequences of PAL in different plants
The identical amino acids are shown in white with black background, the conserved amino acids are shown in black with red background. The conserved active site motif (Ala-Ser-Gly) is indicated by ﬁlled triangles under the sequences, the other active site residues are indicated by open triangle asterisk, the conserved active site motif is denoted by red box. AtPAL: Arabidopsis thaliana (NP_187645.1); VvPAL: Vitis vinifera (RVW44079.1); LrPAL: Lilium regale (ASV46344.1); GbPAL: Ginkgo biloba (ABU49842.1); EaPAL: Equisetum arvense (AAW80639.1); PaPAL: Plagiochasma appendiculatum (AIU99853.1).