半夏PtPAL基因的克隆、表达与酶动力学分析
何潇1,2(
), 刘兴3, 辛正琦1,2, 谢海艳1,2, 辛余凤1,2, 吴能表1,2,*()
), 刘兴3, 辛正琦1,2, 谢海艳1,2, 辛余凤1,2, 吴能表1,2,*()
Molecular cloning, expression, and enzyme kinetic analysis of a phenylalanine ammonia-lyase gene in Pinellia ternate
HE Xiao1,2(), LIU Xing3, XIN Zheng-Qi1,2, XIE Hai-Yan1,2, XIN Yu-Feng1,2, WU Neng-Biao1,2,*()
), LIU Xing3, XIN Zheng-Qi1,2, XIE Hai-Yan1,2, XIN Yu-Feng1,2, WU Neng-Biao1,2,*()
图6. PtPAL重组蛋白时间梯度诱导表达和融合His标签PtPAL重组蛋白纯化
M为蛋白marker。A: PtPAL重组蛋白时间梯度诱导表达; B: 融合His标签PtPAL重组蛋白纯化。
Fig. 6. Time gradient induced of the recombinant PtPAL and its purified His-tagged PtPAL
M: protein molecular weight standards. A: time gradient induced of reconstructive PtPAL; B: the purified His-tagged PtPAL.
