青藏春冬麦区93份小麦地方种质条锈病抗性评价及抗病基因分子鉴定

赵旭阳, 姚方杰, 龙黎, 王昱琦, 康厚扬, 蒋云峰, 李伟, 邓梅, 李豪, 陈国跃

Evaluation of resistance to stripe rust and molecular detection of resistance genes of 93 wheat landraces from the Qinghai-Tibet spring and winter wheat zones

ZHAO Xu-Yang, YAO Fang-Jie, LONG Li, WANG Yu-Qi, KANG Hou-Yang, JIANG Yun-Feng, LI Wei, DENG Mei, LI Hao, CHEN Guo-Yue

表2 Yr基因的侧翼标记及其序列

Table 2 Names and sequences of flanking markers closely linked to Yr genes

Yr 基因 Yr gene 标记类型 Marker type 标记名称 Marker name 引物序列 Primer sequence (5′-3′) 遗传距离 Genetic distance (cM) 参考文献 Reference Yr5 KASP Yr5F GCGCCCCTTTTCGAAAAAATA — Marchal et al. [29] Yr5H CTAGCATCAAACAAGCTAAATA Yr5R ATGTCGAAATATTGCATAACATGG Yr10 Indel Xsdauw79-F TTGCTCTAAGCTGTGGCCT Yuan et al. [30] Xsdauw79-R GAGTTCAACCCCGAACACT Xsdauw79-NF AGAGCCTAAGCGCCTAAGG Xsdauw79-NR TTAAAATCTCCCAAGTACGCA Yr18 STS csLV34 GTTGGTTAAGACTGGTGATGG 0.4 Lagudah et al. [31] TGCTTGCTATTGCTGAATAGT Gene Marker Cssfr5 TTGATGAAACCAGTTTTTTTTCTA — Lagudah et al. [31] GGGAGCATTATTTTTTTCCATCATG ACTTTCCTGAAAATAATACAAGCA TATGCCATTTAACATAATCATGAA Yr24 STS Xwe173 GGGACAAGGGGAGTTGAAGC 1.4 Wang et al. [32] GAGAGTTCCAAGCAGAACAC STS Xwe33 TAAACCAAGTCCCCCAAA — Wang et al. [32] GGAGTCCATCTTCACCGA Yr48 SSR Xwmc727 CATAATCAGGACAGCCGCAC 4.4 Lowe et al. [33] TAGTGGCCTGATGTATCTAGTTGG Yr65 SSR Xgwm18 GGTTGCTGAAGAACCTTATTTAGG 1.2 Cheng et al. [34] TGGCGCCATGATTGCATTATCTTC Yr67 SSR Xbarc182 CCATGGCCAACAGCTCAAGGTCTC 0.4 Xu et al. [35] CGCAAAACCGCATCAGGGAAGCACCAAT SSR Xcfa2040 TCAAATGATTTCAGGTAACCACTA 2.8 Xu et al. [35] TTCCTGATCCCACCAAACAT