小麦多酚氧化酶基因Ppo-A1和Ppo-D1位点等位变异与穗发芽抗性的关系

黄义文, 代旭冉, 刘宏伟, 杨丽, 买春艳, 于立强, 于广军, 张宏军, 李洪杰, 周阳

Relationship between the allelic variations at the Ppo-A1 and Ppo-D1 loci and pre-harvest sprouting resistance in wheat

HUANG Yi-Wen, DAI Xu-Ran, LIU Hong-Wei, YANG Li, MAI Chun-Yan, YU Li-Qiang, YU Guang-Jun, ZHANG Hong-Jun, LI Hong-Jie, ZHOU Yang

表1 Ppo-A1和Ppo-D1位点不同等位变异检测的功能标记信息

Table 1 Information on the functional markers for identifying different alleles at the Ppo-A1 and Ppo-D1 loci

位点 Locus 标记 Marker 引物序列 Primer sequence (5′-3′) 等位基因 Allele 扩增片段 Amplified fragment (bp) 退火温度 Annealing temperature (℃) 参考文献 Reference Ppo-A1 PPO18 F: AACTGCTGGCTCTTCTTCCCA Ppo-A1a 685 60 [25] R: AAGAAGTTGCCCATGTCCGC Ppo-A1b 876 Ppo-D1 PPO16 F: TGCTGACCGACCTTGACTCC Ppo-D1a 713 60 [26] R: CTCGTCACCGTCACCCGTAT PPO29 F: TGAAGCTGCCGGTCATCTAC Ppo-D1b 490 R: AAGTTGCCCATGTCCTCGCC