黄淮麦区小麦粒重基因等位变异的分子鉴定及育种应用

张福彦, 程仲杰, 陈晓杰, 王嘉欢, 陈锋, 范家霖, 张建伟, 杨保安

Molecular identification and breeding application of allelic variation of grain weight gene in wheat from the Yellow-Huai-River Valley

ZHANG Fu-Yan, CHENG Zhong-Jie, CHEN Xiao-Jie, WANG Jia-Huan, CHEN Feng, FAN Jia-Lin, ZHANG Jian-Wei, YANG Bao-An

表1 检测目的基因等位变异的功能标记及其扩增信息

Table 1 Functional markers and amplification information for detecting alleles of target genes in wheat

基因 Genes 等位变异 Alleles 标记引物 Mark primer 引物序列 Primer sequences (5′-3′) 退火温度 Annealing temperature (℃) 扩增片段 Targeted fragment (bp) 参考文献 Reference TaGS-D1 TaGS-D1a GS7D-F AACTTAGGGAGCGAAAACAA 58 562 [7] TaGS-D1b GS7D-R CACCAAGACTGGAGATGAAA 522 [7] TaCwi-A1 TaCwi-A1a CWI22-F GGTGATGAGTTCATGGTTAAT 56 402 [5] CWI22-R AGAAGCCCAACATTAAATCAAC TaCwi-A1b CWI21-F GTGGTGATGAGTTCATGGTTAAG 56 404 [5] CWI21-R AGAAGCCCAACATTAAATCAAC TaGw8-B1 TaGW8-B1a TaGW8-7B-F CGCTCATCCATTCCTTCATCG 60 1097 [15] TaGW8-B1b TaGW8-7B-R GCTATATGGGTTGGTGTCGC 1373 [15]