水稻ptc1隐性核不育系的创制及其配合力分析

李京琳, 李佳林, 李新鹏, 安保光, 曾翔, 吴永忠, 黄培劲, 龙湍

Creation and combining ability analysis of recessive genic sterile lines with a new ptc1 locus in rice

LI Jing-Lin, LI Jia-Lin, LI Xin-Peng, AN Bao-Guang, ZENG Xiang, WU Yong-Zhong, HUANG Pei-Jing, LONG Tuan

图2 ptc1-2的图位克隆 A: ptc1-2位点粗定位在9号染色体长臂的RM7039和RM257两个标记间; B: ptc1-2基因精细定位至D3102和D3676之间, 物理距离为573 bp; C: ptc1-2位点包含从第15,324,556 bp到第15,583,926 bp共259,370 bp缺失, 该缺失片段包含整个PTC1基因。

Fig. 2 Map-based cloning of ptc1-2 A: the ptc1-2 locus was first mapped to an interval between RM7039 and RM257 on the long arm of chromosome 9; B: ptc1-2 was fine mapped to an interval of 573 base pairs between D1380 and D3676; C: the ptc1-2 locus consisted of a 259,370 base-pair deletion from position 15,324,556 base-pair to position 15,583,926 base-pair. The deletion fragment contained the entire coding region of PTC1.