水稻ptc1隐性核不育系的创制及其配合力分析

李京琳, 李佳林, 李新鹏, 安保光, 曾翔, 吴永忠, 黄培劲, 龙湍

Creation and combining ability analysis of recessive genic sterile lines with a new ptc1 locus in rice

LI Jing-Lin, LI Jia-Lin, LI Xin-Peng, AN Bao-Guang, ZENG Xiang, WU Yong-Zhong, HUANG Pei-Jing, LONG Tuan

图3 ptc1-2位点侧翼序列的分离及共分离分析 A: ptc1-2位点两侧基因组序列分离第2轮TAIL-PCR扩增结果; B: ptc1-2突变体的共分离验证。雄性不育表型的F2植株扩增出的产物长546 bp, 而表型正常的F2植株扩增出的产物长811 bp或同时有546 bp和811 bp两条带。M为D2000 marker。

Fig. 3 Isolation of sequences flanking the ptc1-2 locus and co-segregation analysis A: the second round of TAIL-PCR results for isolation of genomic sequences flanking the ptc1-2 locus; B: co-segregation analysis of the ptc1-2 locus with the male sterile phenotype. All PCR products of F2 sterile plants were 546 bp in length. The size of PCR products of F2 fertile plants was either 811 bp, or 546 bp, and 811 bp. M: DNA 2000 bp marker.