大豆种子颜色遗传调控机制研究进展

邱红梅, 陈亮, 侯云龙, 王新风, 陈健, 马晓萍, 崔正果, 张玲, 胡金海, 王跃强, 邱丽娟

Research progress on genetic regulatory mechanism of seed color in soybean (Glycine max)

QIU Hong-Mei, CHEN Liang, HOU Yun-Long, WANG Xin-Feng, CHEN Jian, MA Xiao-Ping, CUI Zheng-Guo, ZHANG Ling, HU Jin-Hai, WANG Yue-Qiang, QIU Li-Juan

表2 调控种子颜色的相关基因信息

Table 2 Genetic information related to seed color regulation

序号 No. 基因编号(v2.1) Gene ID (v2.1) 基因名称 Gene name 英文缩写 Abbreviation 遗传位点 Genetic locus 编码产物注释 Annotation of coding products 参考文献 Reference 1 Glyma.08G109500 查尔酮合成酶1 Chalcone synthase 1 CHS1 I 类黄酮合成途径酶：合成柚皮苷查尔酮 Flavonoid biosynthesis pathway enzyme: synthesis of naringin chalcone Akada et al.[51] 2 Glyma.08G110300 查尔酮合成酶3 Chalcone synthase 3 CHS3 I 类黄酮合成途径酶：合成柚皮苷查尔酮 Flavonoid biosynthesis pathway enzyme: synthesis of naringin chalcone Senda et al.[52] Cho et al.[53] 3 Glyma.08G109200 查尔酮合成酶4 Chalcone synthase 4 CHS4 I 类黄酮合成途径酶：合成柚皮苷查尔酮 Flavonoid biosynthesis pathway enzyme: synthesis of naringin chalcone Liu et al.[54] Tuteja et al.[55] 4 Glyma.11G190900 Argonaute 5蛋白 Argonaute 5 protein AGO5 K1 调控CHS siRNAs在种皮的分布 Regulation of CHS siRNAs distribution in seed coat Rogers et al.[58] Song et al.[59] 5 Glyma.09G025300 内啡肽酶同源物2a Endorphinase homolog 2a DCL2a — 切割含长LIR的转录本,生成22nt siRNA LIR-derived transcripts are predominately processed by GmDCL2 into 22-nt siRNAs Jia et al.[60] 6 Glyma.09G025400 内啡肽酶同源物2b Endorphinase homolog 2a DCL2b — 切割含长LIR的转录本,生成22nt siRNA LIR-derived transcripts are predominately processed by GmDCL2 into 22-nt siRNAs Jia et al.[60] 7 Glyma.06G202300 黄烷酮3’-羟化酶 Flavanone 3’-hydroxylase F3’H T 类黄酮合成途径酶：催化黄烷酮羟基化 Flavonoid biosynthesis pathway enzymes: catalyzing the hydroxylation of flavanones Toda et al.[61] Guo et al.[62] 8 Glyma.13G072100 黄烷酮3’,5’-羟化酶 Flavanone 3’,5’-hydroxylase F3’5’H W1 类黄酮合成途径酶：催化黄烷酮羟基化 Flavonoid biosynthesis pathway enzymes: catalyzing the hydroxylation of flavanones Zabala et al.[33] 9 Glyma.08G062000 花青素还原酶 Anthocyanidin reductase ANR1 O 类黄酮合成途径酶：形成原花青素 Flavonoid biosynthesis pathway enzymes: procyanidins production Yang et al.[32] 10 Glyma.08G062100 花青素还原酶 Anthocyanidin reductase ANR2 O 类黄酮合成途径酶：形成原花青素 Flavonoid biosynthesis pathway enzymes: procyanidins production Yang et al.[32] 11 Glyma.09G235100 R2R3 MYB转录因子 Transcripion foctor R2R3 MYB R2R3 MYB R 转录因子：调控ANS基因的表达 Transcription factors: regulation of ANS gene expression Yan et al.[63] Zabala et al.[64] 12 Glyma.01G198500 CAAX自身免疫蛋白酶 CAAX protease self-immunity CPSI G 防止叶绿素降解使成熟种皮保持绿色 Prevent chlorophyll degradation to keep the mature seed coat green Xie et al.[65] Wang et al.[66] 13 Glyma.01g214600 保持绿色蛋白1 Protein stay-green 1 SGR1 D1 使成熟种子保持绿色 Mature seeds stay-green Fang et al.[34] 宋健[12] Fang et al.[34] Song J[12] 14 Glyma.11g027400 保持绿色蛋白2 Protein stay-green 2 SGR2 D2 使成熟种子保持绿色 Mature seeds stay-green Fang et al.[34] 宋健[12] Fang et al.[34] Song J[12] 15 Glyma.20G241500 查尔酮异构酶1A Chalcone isomerase 1A CHI1A — 类黄酮合成途径酶：催化分子内环化反应 Flavonoid synthesis pathway enzymes: catalyzing intramolecular cyclization Kovinich et al.[63] 16 Glyma.20G241700 查尔酮异构酶2 Chalcone isomerase 2 CHI2 — 类黄酮合成途径酶：催化分子内环化反应 Flavonoid synthesis pathway enzymes: catalyzing intramolecular cyclization Kovinich et al.[63] 17 Glyma.02G048400 黄烷酮3-羟化酶 Naringenin 3-dioxygenase F3H Wp 类黄酮合成途径酶：催化黄烷酮羟基化 Flavonoid biosynthesis pathway enzymes: catalyzing the hydroxylation of flavanones Zabala et al.[64] 18 Glyma.14G072700 二氢黄酮醇还原酶1 Dihydroflavonol 4-reductase 1 DFR1 W3 类黄酮合成途径酶：形成无色花青素 Flavonoid synthesis pathway enzymes: formation of colorless anthocyanins Xu et al.[65] 19 Glyma.17G252200 二氢黄酮醇还原酶2 Dihydroflavonol 4-reductase 2 DFR2 W4 类黄酮合成途径酶：形成无色花青素 Flavonoid synthesis pathway enzymes: formation of colorless anthocyanins Xu et al.[65] 20 Glyma.01G214200 花青素合成酶2 Anthocyanidin synthase 2 ANS2 — 类黄酮合成途径酶：形成花色素 Flavonoid biosynthesis pathway enzymes: anthocyanin formation Kovinich et al.[68] 21 Glyma.11G027700 花青素合成酶3 Anthocyanidin synthase 3 ANS3 — 类黄酮合成途径酶：形成花色素 Flavonoid biosynthesis pathway enzymes: anthocyanin formation Kovinich et al.[68] 22 Glyma.08G066800 UDP类黄酮葡萄糖基转移酶 UDP-flavonoid 3-O-glucosyltransferase UF3GT — 类黄酮合成途径酶：形成花色苷 Flavonoid biosynthesis pathway enzymes: anthocyanin formation Kovinich et al.[68] 23 — 光系统Ⅱ反应中心M亚基 Photosystem II reaction center M subunit Psbm — 具有叶绿素b降解功能 Degradation of chlorophyll b Kohzuma et al.[40]