大豆种子颜色遗传调控机制研究进展

邱红梅, 陈亮, 侯云龙, 王新风, 陈健, 马晓萍, 崔正果, 张玲, 胡金海, 王跃强, 邱丽娟

Research progress on genetic regulatory mechanism of seed color in soybean (Glycine max)

QIU Hong-Mei, CHEN Liang, HOU Yun-Long, WANG Xin-Feng, CHEN Jian, MA Xiao-Ping, CUI Zheng-Guo, ZHANG Ling, HU Jin-Hai, WANG Yue-Qiang, QIU Li-Juan

表3 控制种子颜色等位基因的调控机制

Table 3 Regulation mechanism of alleles controlling seed color

基因/编号 Gene ID 突变基因型 Mutant genotype 种子颜色 Seed color 变异方式 Variation 检测方法 Detection method 参考文献 Reference I Glyma.08G109500 Glyma.08G110300 Glyma.08G109200 ii 黄种皮黑脐 Yellow seed coat with black hilum CHS基因的拷贝数增加 The copy number of CHS gene increased BAC重测序 BAC resequencing Cho et al.[53] ik 鞍挂 Black saddle 基因间序列缺失 Deletion of intergenic sequencesl BAC重测序 BAC resequencing Cho et al.[53] i 深色种皮 Dark pigment seed coat 基因及基因间序列大片段缺失 Large deletions of genes and intergenic sequences BAC重测序 BAC resequencing Cho et al.[53] R Glyma.09G235100 R* 黑种皮 Black seed coat 第2内含子插入CACTA转座子及CACTA序列的甲基化 Insertion of the second intron into the CACTA transposon and methylation of the CACTA sequence 长片段PCR扩增、表达谱分析、甲基化检测 Long fragment PCR amplification, expression profile analysis, methylation detection Zabala et al.[64] r 褐种皮 Brown seed coat 第2外显子单碱基C缺失 Single base C deletion in exon 2 克隆测序 Cloning and sequencing Zabala et al.[64] rm 虎斑 Black spots or concentric rings of black pigment superimposed on an otherwise brown seed coat 第2内含子插入CACTA转座子 Insertion of the second intron into the CACTA transposon 长片段PCR扩增、表达谱分析、甲基化检测 Long fragment PCR amplification, expression profile analysis, methylation detection Zabala et al.[64] T Glyma.06G202300 t 褐种皮 Brown seed coat 第3外显子单碱基A的缺失或非同义突变或单碱基C的缺失 Deletion of single base A or non synonymous mutation or deletion of single base C in exon 3 PCR测序 PCR sequencing Guo et al.[62] tm 深褐和褐色种皮 Dark brown and brown seed coat, 第1内含子插入CACTA转座子 The first intron was inserted into the CACTA transposon 长片段PCR扩增 Long fragment PCR amplification Zabala et al.[31] W1 Glyma.13G072100 w1 淡褐种皮 Buff seed coat 第3外显子插入53 bp The third exon was inserted 53 bp sequence 克隆测序 Cloning and sequencing Guo et al.[62] K1 Glyma.11G190900 k1 鞍挂 Black saddle 第7外显子129 bp的缺失 Deletion of 129 bp sequence in exon 7 转录组与小RNA测序、PCR测序、全基因组深度重测序 Ranscriptome and micro RNA sequencing, PCR sequencing, whole genome deep resequencing Cho et al.[34] G Glyma.01G198500 g 黄种皮 Yellow seed coat 第8内含子末端发生碱基突变 A base mutation occurred at the end of intron 8 克隆测序 Cloning and sequencing Wang et al.[66] D1 Glyma.01g214600 d1 绿种皮、绿子叶 Green seed coat and cotyledon 第2外显子单碱基T缺失 Single base T deletion in exon 2 基因组重测序、克隆测序 Genome resequencing, cloning and sequencing Fang et al.[35], Song J[12] D2 Glyma.11g027400 d2 绿种皮、绿子叶 Green seed coat and cotyledon 322 bp重复插入 322 bp sequence repeated insertion 基因组重测序、克隆测序 Genome resequencing, cloning and sequencing Fang et al.[35], Song J[12] CytG cytG 绿种皮、绿子叶 Green seed coat and cotyledon 5 bp插入 5 bp sequence insertion 对叶绿体基因组广泛的测序 Extensively sequenced the chloroplast genome Kohzuma et al.[40]