红麻DNA甲基化响应镉胁迫及甲基化差异基因的表达分析

卢海, 李增强, 唐美琼, 罗登杰, 曹珊, 岳娇, 胡亚丽, 黄震, 陈涛, 陈鹏

DNA methylation in response to cadmium stress and expression of different methylated genes in kenaf

LU Hai, LI Zeng-Qiang, TANG Mei-Qiong, LUO Deng-Jie, CAO Shan, YUE Jiao, HU Ya-Li, HUANG Zhen, CHEN Tao, CHEN Peng

表1 接头和引物序列

Table 1 Adapter and primers used in this study

引物类型 Primer type 引物名称及序列 Primer name and sequence (5′-3′) EcoR I (E) Hpa II/Msp I (HM) 接头 Adapter EA1 CTCGTAGACTGCGTACC HMA1 GACGATGAGTCTAGAA EA2 AATTGGTACGCAGTC HMA2 CGTTCTAGACTCATC 预扩增 E0 GACTGCGTACCAATTCA HM0 GATGAGTCTAGAACGGT Pre-amplification E1 GACTGCGTACCAATTCAAC HM1 GATGAGTCTAGAACGGTAG E2 GACTGCGTACCAATTCAAG HM2 GATGAGTCTAGAACGGTAC E3 GACTGCGTACCAATTCACA HM3 GATGAGTCTAGAACGGTTG 选择性扩增 E4 GACTGCGTACCAATTCACT HM4 GATGAGTCTAGAACGGTTC Selective amplification E5 GACTGCGTACCAATTCACC HM5 GATGAGTCTAGAACGGTGT E6 GACTGCGTACCAATTCACG HM6 GATGAGTCTAGAACGGTGC E7 GACTGCGTACCAATTCAGC HM7 GATGAGTCTAGAACGGTCT E8 GACTGCGTACCAATTCAGG HM8 GATGAGTCTAGAACGGTCG