红麻DNA甲基化响应镉胁迫及甲基化差异基因的表达分析
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卢海, 李增强, 唐美琼, 罗登杰, 曹珊, 岳娇, 胡亚丽, 黄震, 陈涛, 陈鹏
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DNA methylation in response to cadmium stress and expression of different methylated genes in kenaf
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LU Hai, LI Zeng-Qiang, TANG Mei-Qiong, LUO Deng-Jie, CAO Shan, YUE Jiao, HU Ya-Li, HUANG Zhen, CHEN Tao, CHEN Peng
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表2 实时荧光定量PCR引物序列
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Table 2 Primer sequences for qRT-PCR
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引物名称 Primer name | 正向引物 Forward sequence (5′-3′) | 反向引物 Reverse sequence (5′-3′) | GH3.1 | GACCGCCGTCTGTAACTCG | GGTTCCACAACTCCGCCC | GrKCS1 | GCCGCCAGTGTGATGGATAT | CGGTGCTCGGTTCTCCATT | NADP-ME | TTCATCTTCCCTTCGCCTT | CATCCCAACAACACCTTCCT | ABCG26 | ATTGGTACGCAGTCACGATG | TTCACCTTCATTTGACACGG | L-AAOh | AAGCCTTTCCGATTGTTCTGC | GGAGTTAGATTTTTGTTGTTGGGAG | LRR-RLK | GCATATACACGATAAGAAACCGC | CATGAAGATATTCCAAGCCCC | GaTPPD | CCTTCCTCCCTTGGGTTAGT | TTCTGTTCGCCTGTGGTTTT | XlCGF8.2DB | GACTGCGTACCAATTCAAGGC | AATTGTTGCCATATTTCTCCCAT | His3 (reference gene) | GTGGAGTCAAGAAGCCTCACAG | ATGGCTCTGGAAACGCAAA |
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