红麻DNA甲基化响应镉胁迫及甲基化差异基因的表达分析
卢海, 李增强, 唐美琼, 罗登杰, 曹珊, 岳娇, 胡亚丽, 黄震, 陈涛, 陈鹏

DNA methylation in response to cadmium stress and expression of different methylated genes in kenaf
LU Hai, LI Zeng-Qiang, TANG Mei-Qiong, LUO Deng-Jie, CAO Shan, YUE Jiao, HU Ya-Li, HUANG Zhen, CHEN Tao, CHEN Peng
表6 甲基化差异片段比对分析
Table 6 Comparisons of differentially methylated sequences
序列编号
Sequence number
甲基化差异片段比对结果
Comparisons of differentially methylated sequences
功能注释
Functional annotation
1 Leucine-rich repeat receptor-like kinases, (LRR-RLK) At5g15730, Gossypium 充当胞外信号的受体, 参与各种环境及发育信号的感知和传递[22]
As the receptor of extracellular signal, it participates in the perception and transmission of various environmental and developmental signals[22].
2 Zinc finger protein XlCGF8.2DB-like
(XlCGF8.2DB), Bemisia tabaci gastrula
参与一些重要的调控过程, 如; 形态建成、花粉发育、胚发育、胁迫反应等[23]
Widely involved in regulatory of important processes, such as morphogenesis, pollen development, embryo development, stress response, and so on[23].
3 Ankyrin-3-like, Gossypium arboreum 尚未见报道。
Has not been reported.
4 Trehalose-phosphate phosphatase D (GaTPPD), Gossypium arboreum 海藻糖磷酸磷酸酶在植物生长和胁迫反应中起重要作用[24]
Trehalose phosphatase plays an important role in plant growth and stress response[24].
5 Serine/threonine-protein kinase, Gossypium raimondii 丝氨酸/苏氨酸蛋白激酶, 催化多种功能蛋白的磷酸化, 调控植物非生物胁迫[25]
Serine/threonine protein kinases, catalyze the phosphorylation of various functional proteins and regulate abiotic stress in plants[25].
6 Peroxidasin (Pxdn) transcript variant X6, Castor canadensis 过氧化物酶, 植物抗逆过程中的关键酶之一[26]
Peroxidase is one of the key enzymes in plant stress resistance[26].
7 Protein NRT1/ PTR FAMILY 2.7 (NPF2.7), Gossypium hirsutum NRT1/PTR家族蛋白参与转运植物激素及次生代谢物合成过程。
NRT1/PTR family proteins is involved in the transport of plant hormones and the synthesis of secondary metabolites.
8 60S ribosomal protein L16(RPL16), Gossypium hirsutum 参与细胞中蛋白质合成及调控基因表达[27]
It is involved in protein synthesis and gene expression regulation[27].
9 Receptor-like protein kinase At2g46850, Durio zibethinus 类受体激酶通过接收和传递胞外信号调控细胞的生理反应, 参与植物生长发育过程。
Receptor like kinases are involved in plant growth and development by receiving and transmitting extracellular signals to regulate cell physiological responses.
10 Transcript variant X2, Gossypium raimondii 转录变异体X2, 参与细胞增殖及细胞周期活动的调节。
Transcription variant X2 is involved in the regulation of cell proliferation and cell cycle activity.
11 3-ketoacyl-CoA synthase 1 (GrKCS1), Gossypium raimondii 3-酮脂酰辅酶A合成酶, 在抵抗干旱和盐害等非生物胁迫过程中起着重要的作用。
3-ketoacyl coenzyme A synthetase plays an important role in abiotic stresses such as drought and salt stress.
12 L-ascorbate oxidase homolog (L-AAOh), Gossypium hirsutum 抗坏血酸氧化酶, 在调节植物发育过程和应激反应方面发挥重要作用[21]
L-Ascorbic acid oxidase plays an important role in regulating plant development and stress response[21].
13 Indole-3-acetic acid-amido synthetase (GH3.1), Camellia sinensis 吲哚-3醋酸-酰胺合成酶, 通过调节植物体内激素的动态平衡, 参与调控植物的生长发育过程[18]
Indole-3 acetate amide synthetase is involved in the regulation of plant growth and development by regulating the dynamic balance of hormones in plants[18].
14 Acyl-CoA-binding domain-containing protein 4 (ACBD4) 酰基辅酶A结合域蛋白, 在植物的生长发育、生物和非生物胁迫应答中起重要的作用。
Acyl-CoA-binding domain proteins play an important role in plant growth and development, biological and abiotic stress response.
序列编号
Sequence number
甲基化差异片段比对结果
Comparisons of differentially methylated sequences
功能注释
Functional annotation
15 ABC transporter G family member 26 (ABCG26), Gossypium arboreum ABC 转运蛋白G家族参与植物信号转导、次生代谢物运输和非生物胁迫响应等过程[20]
ABC transporter G family are involved in plant signal transduction, secondary metabolite transport and abiotic stress response[20].
16 NADP-dependent malic enzyme (NADP-ME) At1G65930, Gossypium hirsutum NADP依赖苹果酸酶, 通过苹果酸代谢来平衡细胞内的pH来防御生物和非生物胁迫[19]
NADP relies on malic acid enzyme to balance intracellular pH through malate metabolism to protect against biotic and abiotic stresses[19].
17 NAC domain-containing protein 71 (GrNAC71) NAC转录因子, 在逆境胁迫信号转导过程中发挥重要作用[28]
NAC transcription factor plays an important role in stress signal transduction[28].
18 F-box protein At3g07870, Gossypium raimondii 参与调控胞内蛋白降解、受体识别和信号传导[29]
It is involved in the regulation of intracellular protein degradation, receptor recognition and signal transduction[29].
19 Pentatricopeptide repeat-containing
protein (PPRC) At5g55740, Gossypium arboreum
从RNA剪切、编辑、降解和翻译等多个方面影响细胞器中RNA的新陈代谢[30]
RNA metabolism in organelles is affected by RNA splicing, editing, degradation, and translation[30].
20 Ethylene-responsive transcription factor 2 (ERF2) 乙烯转录因子(AP2/ERFs)调控激素响应非生物胁迫[31]
Ethylene transcription factors (AP2/ERFs), widely involved in plant hormone regulation and thus response to abiotic stress[31].