红麻DNA甲基化响应镉胁迫及甲基化差异基因的表达分析

卢海, 李增强, 唐美琼, 罗登杰, 曹珊, 岳娇, 胡亚丽, 黄震, 陈涛, 陈鹏

DNA methylation in response to cadmium stress and expression of different methylated genes in kenaf

LU Hai, LI Zeng-Qiang, TANG Mei-Qiong, LUO Deng-Jie, CAO Shan, YUE Jiao, HU Ya-Li, HUANG Zhen, CHEN Tao, CHEN Peng

图2 MSAP聚丙烯酰胺凝胶电泳图 泳道M代表BM50 DNA marker; 泳道1和3代表EcoR I/Hpa II酶切; 泳道2和4代表EcoR I/Msp I酶切。1和2: 0 μmol L-1 CdCl2; 3和4: 300 μmol L-1 CdCl2。蓝色方框; I型(无甲基化); 黑色方框; II型(半甲基化); 白色方框; III型(全甲基化); 红色方框; IV型(全甲基化)。

Fig. 2 MSAP acrylamide gel detection Lane M: BM50 DNA marker; Lanes 1 and 3 represent digestion with EcoR I/Hpa II; Lanes 2 and 4 represent digestion with EcoR I/Msp I. Lanes 1, 2 and 3, 4 represent CdCl2 concentration of 0 μmol L-1 and 300 μmol L-1, respectively. The blue frame, black frame, white frame, and red frame represent the type I (no methylation), type II (hemi-methylation), type III (full methylation), and type IV (full methylation), respectively.