病毒介导的GFP-ATG8在小麦上的表达和在自噬活性监测上的应用

胡蕊洁, 杨向芸, 贾磊, 李玉如, 项月, 岳洁瑜, 王华忠

Virus-mediated expression of GFP-ATG8 for autophagy monitoring in wheat

HU Rui-Jie, YANG Xiang-Yun, JIA Lei, LI Yu-Ru, XIANG Yue, YUE Jie-Yu, WANG Hua-Zhong

图2 FoMV介导的GFP和GFP-TaATG8a在烟草上的表达 将GFP或GFP-TaATG8a的VOX载体导入农杆菌, 采用Agroinfiltration方法转化烟草叶片。对照(CK)烟草叶片注射不含农杆菌的MMA溶液。转化7 d后在荧光显微镜下观察GFP荧光。标尺为100 μm。

Fig. 2 FoMV-mediated expression of GFP and GFP-TaATG8a in N. benthamiana leaves The GFP-expressing and the GFP-TaATG8a-expressing vector plasmids were transformed into Agrobacterium tumefaciens and agroinfiltrated into N. benthamiana leaves, respectively. Control N. benthamiana leaves were infiltrated with the MMA buffer. GFP fluorescence in N. benthamiana leaves was observed at 7 days after agroinfiltration. Bar: 100 μm.