病毒介导的GFP-ATG8在小麦上的表达和在自噬活性监测上的应用

胡蕊洁, 杨向芸, 贾磊, 李玉如, 项月, 岳洁瑜, 王华忠

Virus-mediated expression of GFP-ATG8 for autophagy monitoring in wheat

HU Rui-Jie, YANG Xiang-Yun, JIA Lei, LI Yu-Ru, XIANG Yue, YUE Jie-Yu, WANG Hua-Zhong

图3 FoMV介导的GFP和GFP-TaATG8a在小麦叶片中的表达 将表达GFP和GFP-TaATG8a的烟草叶片汁液摩擦接种于二叶期小麦幼苗第2叶, 对照(CK)植株摩擦的汁液来自注射MMA溶液的对照烟草植株叶片。分别于接种后第7天和20天观察第2叶和第3叶中的GFP荧光。标尺为500 μm。

Fig. 3 FoMV-mediated expression of GFP and GFP-TaATG8a in wheat leaf tissues The second leaves of two-leaf-stage wheat seedlings were rub-inoculated with extract prepared from GFP-expressing or GFP-TaATG8a-expressing N. benthamiana leaves containing FoMV virions. For control seedlings, their second leaves were rub-inoculated with extract prepared from N. benthamiana leaves infiltrated with the MMA buffer. GFP fluorescence in the inoculated leaves was observed at 7 days and that in the third uninoculated leaves observed at 20 days after virus inoculation. Bar: 500 μm.